{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Molitor R"],"funding":["Horizon 2020"],"pagination":["274-284"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6922526"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Hydrolases acting on polyesters like cutin, polycaprolactone or polyethylene terephthalate (PET) are of interest for several biotechnological applications like waste treatment, biocatalysis and sustainable polymer modifications. Recent studies suggest that a large variety of such enzymes are still to be identified and explored in a variety of microorganisms, including bacteria of the genus Pseudomonas. For activity-based screening, methods have been established using agar plates which contain nanoparticles of polycaprolactone or PET prepared by solvent precipitation and evaporation. In this protocol article, we describe a straightforward agar plate-based method using emulsifiable artificial polyesters as substrates, namely Impranil<sup>®</sup> DLN and liquid polycaprolactone diol (PLD). Th"],"journal":["Microbial biotechnology"],"pubmed_title":["Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species."],"pmcid":["PMC6922526"],"funding_grant_id":["634486"],"pubmed_authors":["Loeschcke A","Jaeger KE","Molitor R","Bollinger A","Kubicki S","Thies S"],"additional_accession":[]},"is_claimable":false,"name":"Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species.","description":"Hydrolases acting on polyesters like cutin, polycaprolactone or polyethylene terephthalate (PET) are of interest for several biotechnological applications like waste treatment, biocatalysis and sustainable polymer modifications. Recent studies suggest that a large variety of such enzymes are still to be identified and explored in a variety of microorganisms, including bacteria of the genus Pseudomonas. For activity-based screening, methods have been established using agar plates which contain nanoparticles of polycaprolactone or PET prepared by solvent precipitation and evaporation. In this protocol article, we describe a straightforward agar plate-based method using emulsifiable artificial polyesters as substrates, namely Impranil<sup>®</sup> DLN and liquid polycaprolactone diol (PLD). Th","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jan","modification":"2025-04-05T16:21:39.462Z","creation":"2020-05-22T01:04:05Z"},"accession":"S-EPMC6922526","cross_references":{"pubmed":["31016871"],"doi":["10.1111/1751-7915.13418"]}}