biostudies-literatureMolitor RHorizon 2020274-284https://www.ebi.ac.uk/biostudies/studies/S-EPMC6922526biostudies-literatureUnknown13(1)Hydrolases acting on polyesters like cutin, polycaprolactone or polyethylene terephthalate (PET) are of interest for several biotechnological applications like waste treatment, biocatalysis and sustainable polymer modifications. Recent studies suggest that a large variety of such enzymes are still to be identified and explored in a variety of microorganisms, including bacteria of the genus Pseudomonas. For activity-based screening, methods have been established using agar plates which contain nanoparticles of polycaprolactone or PET prepared by solvent precipitation and evaporation. In this protocol article, we describe a straightforward agar plate-based method using emulsifiable artificial polyesters as substrates, namely Impranil^® DLN and liquid polycaprolactone diol (PLD). ThMicrobial biotechnologyAgar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species.PMC6922526634486Loeschcke AJaeger KEMolitor RBollinger AKubicki SThies SfalseAgar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species.Hydrolases acting on polyesters like cutin, polycaprolactone or polyethylene terephthalate (PET) are of interest for several biotechnological applications like waste treatment, biocatalysis and sustainable polymer modifications. Recent studies suggest that a large variety of such enzymes are still to be identified and explored in a variety of microorganisms, including bacteria of the genus Pseudomonas. For activity-based screening, methods have been established using agar plates which contain nanoparticles of polycaprolactone or PET prepared by solvent precipitation and evaporation. In this protocol article, we describe a straightforward agar plate-based method using emulsifiable artificial polyesters as substrates, namely Impranil^® DLN and liquid polycaprolactone diol (PLD). Th2020-01-01T00:00:00Z2020 Jan2025-04-05T16:21:39.462Z2020-05-22T01:04:05ZS-EPMC69225263101687110.1111/1751-7915.13418