biostudies-literatureUnknown10Weber REHorizon 2020 Framework ProgrammeOne of the most demanding challenges in infection control is the worldwide dissemination of multidrug-resistant (MDR) bacteria in clinical settings. Especially the increasing prevalence of carbapenemase producing Gram-negative pathogens poses an urgent threat to public health, as these enzymes confer resistance to almost all β-lactam antibiotics including carbapenems. In this study, we report a prolonged nosocomial outbreak of various NDM-1-producing <i>Enterobacterales</i> species due to clonal spread and cross-species exchange of plasmids and possibly transposons. Between July 2015 and September 2017, a total of 51 carbapenemase-positive isolates were collected from 38 patients and three environmental sources in a single German hospital. Combining molecular typing methods and whole genomFrontiers in microbiology2817https://www.ebi.ac.uk/biostudies/studies/S-EPMC6929489biostudies-literatureIS<i>26</i>-Mediated Transfer of <i>bla</i> _NDM-1 as the Main Route of Resistance Transmission During a Polyclonal, Multispecies Outbreak in a German Hospital.PMC6929489Pietsch MGatermann SWeber RELuft DPfennigwerth NPfeifer YMartin MKaase MFuchs SFruhauf AWerner GfalseIS<i>26</i>-Mediated Transfer of <i>bla</i> _NDM-1 as the Main Route of Resistance Transmission During a Polyclonal, Multispecies Outbreak in a German Hospital.One of the most demanding challenges in infection control is the worldwide dissemination of multidrug-resistant (MDR) bacteria in clinical settings. Especially the increasing prevalence of carbapenemase producing Gram-negative pathogens poses an urgent threat to public health, as these enzymes confer resistance to almost all β-lactam antibiotics including carbapenems. In this study, we report a prolonged nosocomial outbreak of various NDM-1-producing <i>Enterobacterales</i> species due to clonal spread and cross-species exchange of plasmids and possibly transposons. Between July 2015 and September 2017, a total of 51 carbapenemase-positive isolates were collected from 38 patients and three environmental sources in a single German hospital. Combining molecular typing methods and whole genom2019-01-01T00:00:00Z20192026-05-04T09:02:13.545Z2020-05-22T07:37:49ZS-EPMC69294893192101510.3389/fmicb.2019.02817