biostudies-literatureChang FPChinese Medicine Research Center, China Medical UniversityTaiwan Ministry of Health and Welfare Clinical Trial CenterE4271https://www.ebi.ac.uk/biostudies/studies/S-EPMC6930649biostudies-literatureUnknown24(23)One new iridoid, namely neonanin C (<b>1</b>) one monocyclic iridoid ring-opened derivative namely neonanin D <b>(2)</b>, two new bis-iridoid derivatives namely reticunin A (<b>3</b>) and reticunin B (<b>4</b>) with sixteen known compounds (<b>5</b>-<b>20</b>) were isolated from the stems of <i>Neonauclea reticulata</i> (Havil.) Merr. These new structures were determined by the detailed analysis of spectroscopic data and comparison with the data of known analogues. Compounds <b>1</b>-<b>20</b> were evaluated for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages cell line. The results showed that all compounds exhibited no obvious cytotoxicity compared to the control group and five compounds including isoboonein (<b>7</b>), syringaresinol (<b>10</b>), (+)-medioresinol (<b>12</b>), protocatechuic acid (<b>14</b>) and <i>trans</i>-caffeic acid (<b>15</b>) exhibited inhibitory activities with IC₅₀ values at 86.27 ± 3.45; 9.18 ± 1.90; 76.18 ± 2.42; 72.91 ± 4.97 and 95.16 ± 1.20 µg/mL, respectively.Molecules (Basel, Switzerland)Four New Iridoid Metabolites Have Been Isolated from the Stems of <i>Neonauclea reticulata</i> (Havil.) Merr. with Anti-Inflammatory Activities on LPS-Induced RAW264.7 Cells.PMC6930649CMRC-CHM-4MOHW108-TDU-B-212-133004Huang SSKuo TFKuo YHLee THHuang GJChang CIChang FPfalseFour New Iridoid Metabolites Have Been Isolated from the Stems of <i>Neonauclea reticulata</i> (Havil.) Merr. with Anti-Inflammatory Activities on LPS-Induced RAW264.7 Cells.One new iridoid, namely neonanin C (<b>1</b>) one monocyclic iridoid ring-opened derivative namely neonanin D <b>(2)</b>, two new bis-iridoid derivatives namely reticunin A (<b>3</b>) and reticunin B (<b>4</b>) with sixteen known compounds (<b>5</b>-<b>20</b>) were isolated from the stems of <i>Neonauclea reticulata</i> (Havil.) Merr. These new structures were determined by the detailed analysis of spectroscopic data and comparison with the data of known analogues. Compounds <b>1</b>-<b>20</b> were evaluated for inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages cell line. The results showed that all compounds exhibited no obvious cytotoxicity compared to the control group and five compounds including isoboonein (<b>7</b>), syringaresinol (<b>10</b>), (+)-medioresinol (<b>12</b>), protocatechuic acid (<b>14</b>) and <i>trans</i>-caffeic acid (<b>15</b>) exhibited inhibitory activities with IC₅₀ values at 86.27 ± 3.45; 9.18 ± 1.90; 76.18 ± 2.42; 72.91 ± 4.97 and 95.16 ± 1.20 µg/mL, respectively.2019-01-01T00:00:00Z2019 Nov2025-04-05T16:21:19.542Z2020-05-22T00:46:23ZS-EPMC69306493177118610.3390/molecules24234271