biostudies-literatureRabelo MSNIDCR NIH HHSFoundation for the National Institutes of HealthFundação de Amparo à Pesquisa do Estado de São Paulo2020-2029https://www.ebi.ac.uk/biostudies/studies/S-EPMC6933074biostudies-literatureUnknown25(8)<h4>Objective</h4>To compare the myeloid and plasmacytoid DC counts and maturation status among subjects with/without generalized periodontitis (GP) and type 2 diabetes mellitus (T2DM).<h4>Methods</h4>The frequency and maturation status of myeloid and plasmacytoid blood DCs were analyzed by flow cytometry in four groups of 15 subjects: healthy controls, T2DM with generalized CP (T2DM + GP), prediabetes with GP (PD + GP), and normoglycemics with GP (NG + GP). RT-PCR was used to determine levels of Porphyromonas gingivalis in the oral biofilms and within panDCs. The role of exogenous glucose effects on differentiation and apoptosis of healthy human MoDCs was explored in vitro.<h4>Results</h4>Relative to controls and to NG + GP, T2DM + GP showed significantly lower CD1c + and CD303 + DC countOral diseasesInfluence of T2DM and prediabetes on blood DC subsets and function in subjects with periodontitis.PMC69330742011/06982-4R01 DE0143282011/06982‐4R01 DE014328 &amp; R21 DE020916R01 DE014328 & R21 DE020916R21 DE020916El-Awady AMoura Foz ARomito GAHisse Gomes GRajendran MLowry SCutler CWMeghil MMSusin CRabelo MSfalseInfluence of T2DM and prediabetes on blood DC subsets and function in subjects with periodontitis.<h4>Objective</h4>To compare the myeloid and plasmacytoid DC counts and maturation status among subjects with/without generalized periodontitis (GP) and type 2 diabetes mellitus (T2DM).<h4>Methods</h4>The frequency and maturation status of myeloid and plasmacytoid blood DCs were analyzed by flow cytometry in four groups of 15 subjects: healthy controls, T2DM with generalized CP (T2DM + GP), prediabetes with GP (PD + GP), and normoglycemics with GP (NG + GP). RT-PCR was used to determine levels of Porphyromonas gingivalis in the oral biofilms and within panDCs. The role of exogenous glucose effects on differentiation and apoptosis of healthy human MoDCs was explored in vitro.<h4>Results</h4>Relative to controls and to NG + GP, T2DM + GP showed significantly lower CD1c + and CD303 + DC count2019-01-01T00:00:00Z2019 Nov2026-05-01T00:49:26.696Z2020-11-03T08:05:10ZS-EPMC69330743154151610.1111/odi.13200