{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Saleem S"],"pubmed_abstract":["<h4>Background</h4>The aim of this pilot study was to identify proteins associated with advancement of colon cancer (CC).<h4>Methods</h4>A quantitative proteomics approach was used to determine the global changes in the proteome of primary colon cancer from patients with non-cancer normal colon (NC), non-adenomatous colon polyp (NAP), non-metastatic tumor (CC NM) and metastatic tumor (CC M) tissues, to identify up- and down-regulated proteins. Total protein was extracted from each biopsy, trypsin-digested, iTRAQ-labeled and the resulting peptides separated using strong cation exchange (SCX) and reverse-phase (RP) chromatography on-line to electrospray ionization mass spectrometry (ESI-MS).<h4>Results</h4>Database searching of the MS/MS data resulted in the identification of 2777 proteins w"],"journal":["Clinical proteomics"],"pagination":["44"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6935225"],"repository":["biostudies-literature"],"pubmed_title":["Proteomics analysis of colon cancer progression."],"pmcid":["PMC6935225"],"pubmed_authors":["Atiq A","Khattak S","Sadr-Ul Shaheed","Syed AA","Ahmad AH","Sutton C","Tariq S","Tahseen M","Hassan S","Saleem S","Yusuf MA","Abu Bakar M","Aleem I","Hussain M"],"additional_accession":[]},"is_claimable":false,"name":"Proteomics analysis of colon cancer progression.","description":"<h4>Background</h4>The aim of this pilot study was to identify proteins associated with advancement of colon cancer (CC).<h4>Methods</h4>A quantitative proteomics approach was used to determine the global changes in the proteome of primary colon cancer from patients with non-cancer normal colon (NC), non-adenomatous colon polyp (NAP), non-metastatic tumor (CC NM) and metastatic tumor (CC M) tissues, to identify up- and down-regulated proteins. Total protein was extracted from each biopsy, trypsin-digested, iTRAQ-labeled and the resulting peptides separated using strong cation exchange (SCX) and reverse-phase (RP) chromatography on-line to electrospray ionization mass spectrometry (ESI-MS).<h4>Results</h4>Database searching of the MS/MS data resulted in the identification of 2777 proteins w","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-05-03T07:53:14.689Z","creation":"2020-05-22T07:00:36Z"},"accession":"S-EPMC6935225","cross_references":{"pubmed":["31889941"],"doi":["10.1186/s12014-019-9264-y"]}}