biostudies-literatureUnknown16Saleem S<h4>Background</h4>The aim of this pilot study was to identify proteins associated with advancement of colon cancer (CC).<h4>Methods</h4>A quantitative proteomics approach was used to determine the global changes in the proteome of primary colon cancer from patients with non-cancer normal colon (NC), non-adenomatous colon polyp (NAP), non-metastatic tumor (CC NM) and metastatic tumor (CC M) tissues, to identify up- and down-regulated proteins. Total protein was extracted from each biopsy, trypsin-digested, iTRAQ-labeled and the resulting peptides separated using strong cation exchange (SCX) and reverse-phase (RP) chromatography on-line to electrospray ionization mass spectrometry (ESI-MS).<h4>Results</h4>Database searching of the MS/MS data resulted in the identification of 2777 proteins wClinical proteomics44https://www.ebi.ac.uk/biostudies/studies/S-EPMC6935225biostudies-literatureProteomics analysis of colon cancer progression.PMC6935225Atiq AKhattak SSadr-Ul ShaheedSyed AAAhmad AHSutton CTariq STahseen MHassan SSaleem SYusuf MAAbu Bakar MAleem IHussain MfalseProteomics analysis of colon cancer progression.<h4>Background</h4>The aim of this pilot study was to identify proteins associated with advancement of colon cancer (CC).<h4>Methods</h4>A quantitative proteomics approach was used to determine the global changes in the proteome of primary colon cancer from patients with non-cancer normal colon (NC), non-adenomatous colon polyp (NAP), non-metastatic tumor (CC NM) and metastatic tumor (CC M) tissues, to identify up- and down-regulated proteins. Total protein was extracted from each biopsy, trypsin-digested, iTRAQ-labeled and the resulting peptides separated using strong cation exchange (SCX) and reverse-phase (RP) chromatography on-line to electrospray ionization mass spectrometry (ESI-MS).<h4>Results</h4>Database searching of the MS/MS data resulted in the identification of 2777 proteins w2019-01-01T00:00:00Z20192026-05-03T07:53:14.689Z2020-05-22T07:00:36ZS-EPMC69352253188994110.1186/s12014-019-9264-y