{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Meng S"],"funding":["National Key R&amp;D Program of China","the CAMS Innovation Fund for Medical Sciences"],"pagination":["8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6935500"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17"],"pubmed_abstract":["<h4>Background</h4>ALKBH7 is a mitochondrial protein, involved in programmed necrosis, fatty acid metabolism, cell cycle regulation, and prostate cancer disease. However, the exact roles of ALKBH7 and the underlying molecular mechanisms remain mysterious. Thus, investigations of the interactome and proteomic responses of ALKBH7 in cell lines using proteomics strategies are urgently required.<h4>Methods</h4>In the present study, we investigated the interactome of ALKBH7 in mitochondria through immunoprecipitation-mass spectrometry/mass spectrometry (IP-MS/MS). Additionally, we established the ALKBH7 knockdown and overexpression cell lines and further identified the differentially expressed proteins (DEPs) in these cell lines by TMT-based MS/MS. Two DEPs (UQCRH and HMGN1) were validated by w"],"journal":["Proteome science"],"pubmed_title":["The interactome and proteomic responses of ALKBH7 in cell lines by in-depth proteomics analysis."],"pmcid":["PMC6935500"],"funding_grant_id":["2018YFA0108600","2016-I2M-1-004"],"pubmed_authors":["Meng S","Dou W","Ge W","Zhan S"],"additional_accession":[]},"is_claimable":false,"name":"The interactome and proteomic responses of ALKBH7 in cell lines by in-depth proteomics analysis.","description":"<h4>Background</h4>ALKBH7 is a mitochondrial protein, involved in programmed necrosis, fatty acid metabolism, cell cycle regulation, and prostate cancer disease. However, the exact roles of ALKBH7 and the underlying molecular mechanisms remain mysterious. Thus, investigations of the interactome and proteomic responses of ALKBH7 in cell lines using proteomics strategies are urgently required.<h4>Methods</h4>In the present study, we investigated the interactome of ALKBH7 in mitochondria through immunoprecipitation-mass spectrometry/mass spectrometry (IP-MS/MS). Additionally, we established the ALKBH7 knockdown and overexpression cell lines and further identified the differentially expressed proteins (DEPs) in these cell lines by TMT-based MS/MS. Two DEPs (UQCRH and HMGN1) were validated by w","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019","modification":"2026-05-01T01:19:03.398Z","creation":"2020-05-22T01:09:14Z"},"accession":"S-EPMC6935500","cross_references":{"pubmed":["31889914"],"doi":["10.1186/s12953-019-0156-x"]}}