biostudies-literatureVadillo EConsejo Nacional de Ciencia y TecnologíaRoyal Society26752-26758https://www.ebi.ac.uk/biostudies/studies/S-EPMC6936681biostudies-literatureUnknown116(52)Neutrophil extravasation is a migratory event in response to inflammation that depends on cytoskeletal dynamics regulated by myosins. Myosin-1e (Myo1e) is a long-tailed class-I myosin that has not yet been studied in the context of neutrophil-endothelial interactions and neutrophil extravasation. Intravital microscopy of TNFα-inflamed cremaster muscles in Myo1e-deficient mice revealed that Myo1e is required for efficient neutrophil extravasation. Specifically, Myo1e deficiency caused increased rolling velocity, decreased firm adhesion, aberrant crawling, and strongly reduced transmigration. Interestingly, we observed a striking discontinuous rolling behavior termed "intermittent rolling," during which Myo1e-deficient neutrophils showed alternating rolling and jumping movements. SurprisinglProceedings of the National Academy of Sciences of the United States of AmericaIntermittent rolling is a defect of the extravasation cascade caused by Myosin1e-deficiency in neutrophils.PMC6936681284292NAF/R1/180017Chanez-Paredes SGuerrero-Fonseca IMVargas-Robles HGiron-Perez DAGarcia-Ponce ASchnoor MNava PVadillo ESantos-Argumedo LCastellanos-Martinez RfalseIntermittent rolling is a defect of the extravasation cascade caused by Myosin1e-deficiency in neutrophils.Neutrophil extravasation is a migratory event in response to inflammation that depends on cytoskeletal dynamics regulated by myosins. Myosin-1e (Myo1e) is a long-tailed class-I myosin that has not yet been studied in the context of neutrophil-endothelial interactions and neutrophil extravasation. Intravital microscopy of TNFα-inflamed cremaster muscles in Myo1e-deficient mice revealed that Myo1e is required for efficient neutrophil extravasation. Specifically, Myo1e deficiency caused increased rolling velocity, decreased firm adhesion, aberrant crawling, and strongly reduced transmigration. Interestingly, we observed a striking discontinuous rolling behavior termed "intermittent rolling," during which Myo1e-deficient neutrophils showed alternating rolling and jumping movements. Surprisingl2019-01-01T00:00:00Z2019 Dec2026-05-06T22:28:52.142Z2026-04-07T22:28:14.834ZS-EPMC69366813181102510.1073/pnas.1902502116