{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Surmiak M"],"funding":["National Centre of Science in Poland","Swiss National Science Foundation"],"pagination":["1-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6939603"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["61(1)"],"pubmed_abstract":["Activation of neutrophils is an important mechanism in the pathology of granulomatosis with polyangiitis (GPA). In this study, we evaluated whether extracellular vesicles (EVs) circulating in the plasma of GPA patients could contribute to this process. EVs from the plasma of GPA patients in the active stage of the disease (n = 10) and healthy controls (n = 10) were isolated by ultracentrifugation and characterized by flow cytometry (CD63, CD8) and nanoparticle tracking analysis. Targeted oxylipin lipidomics of EVs was performed by HPLC-MS/MS. EV/oxylipin-induced neutrophil extracellular traps (NETs) were analyzed by confocal microscopy, and released double-stranded DNA (dsDNA) was quantified by PicoGreen fluorescent dye. Reactive oxygen species (ROS) production and neutrophils' EV binding/"],"journal":["Journal of lipid research"],"pubmed_title":["LTB<sub>4</sub> and 5-oxo-ETE from extracellular vesicles stimulate neutrophils in granulomatosis with polyangiitis."],"pmcid":["PMC6939603"],"funding_grant_id":["31003A_173215","173215","2016/21/D/NZ6/02123","310030_166473"],"pubmed_authors":["Sanak M","Gielicz A","Yousefi S","Surmiak M","Wawrzycka-Adamczyk K","Simon HU","Szatanek R","Stojkov D"],"additional_accession":[]},"is_claimable":false,"name":"LTB<sub>4</sub> and 5-oxo-ETE from extracellular vesicles stimulate neutrophils in granulomatosis with polyangiitis.","description":"Activation of neutrophils is an important mechanism in the pathology of granulomatosis with polyangiitis (GPA). In this study, we evaluated whether extracellular vesicles (EVs) circulating in the plasma of GPA patients could contribute to this process. EVs from the plasma of GPA patients in the active stage of the disease (n = 10) and healthy controls (n = 10) were isolated by ultracentrifugation and characterized by flow cytometry (CD63, CD8) and nanoparticle tracking analysis. Targeted oxylipin lipidomics of EVs was performed by HPLC-MS/MS. EV/oxylipin-induced neutrophil extracellular traps (NETs) were analyzed by confocal microscopy, and released double-stranded DNA (dsDNA) was quantified by PicoGreen fluorescent dye. Reactive oxygen species (ROS) production and neutrophils' EV binding/","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jan","modification":"2026-06-17T06:16:20.624Z","creation":"2021-02-20T17:20:15Z"},"accession":"S-EPMC6939603","cross_references":{"pubmed":["31740445"],"doi":["10.1194/jlr.m092072","10.1194/jlr.M092072"]}}