{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Martins AN"],"funding":["Intramural NIH HHS"],"pagination":["269-283"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6941940"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1632"],"pubmed_abstract":["Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV replication in human cells."],"journal":["Methods in molecular biology (Clifton, N.J.)"],"pubmed_title":["Intracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells."],"pmcid":["PMC6941940"],"funding_grant_id":["ZIA BC011720-02"],"pubmed_authors":["Ke W","Jawahar V","Striplin C","Afonin KA","Martins AN","Freed EO","Striplin M"],"additional_accession":[]},"is_claimable":false,"name":"Intracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells.","description":"Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV replication in human cells.","dates":{"release":"2017-01-01T00:00:00Z","publication":"2017","modification":"2024-11-13T04:54:10.889Z","creation":"2020-05-22T07:07:08Z"},"accession":"S-EPMC6941940","cross_references":{"pubmed":["28730446"],"doi":["10.1007/978-1-4939-7138-1_18"]}}