biostudies-literatureMartins ANIntramural NIH HHS269-283https://www.ebi.ac.uk/biostudies/studies/S-EPMC6941940biostudies-literatureUnknown1632Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV replication in human cells.Methods in molecular biology (Clifton, N.J.)Intracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells.PMC6941940ZIA BC011720-02Ke WJawahar VStriplin CAfonin KAMartins ANFreed EOStriplin MfalseIntracellular Reassociation of RNA-DNA Hybrids that Activates RNAi in HIV-Infected Cells.Human immunodeficiency virus Type 1 (HIV-1) is the major cause of acquired immune deficiency syndrome (AIDS). In 2014, it was estimated that 1.2 million people died from AIDS-related illnesses. RNA interference-based therapy to block HIV replication is a field that, as of now, is without any FDA-approved drugs available for clinical use. In this chapter we describe a protocol for testing and utilizing a new approach that relies on reassociation of RNA-DNA hybrids activating RNAi and blocking HIV replication in human cells.2017-01-01T00:00:00Z20172024-11-13T04:54:10.889Z2020-05-22T07:07:08ZS-EPMC69419402873044610.1007/978-1-4939-7138-1_18