biostudies-literatureMengsteab PYNational Institute of Arthritis and Musculoskeletal and Skin DiseasesNIAMS NIH HHSe0227181https://www.ebi.ac.uk/biostudies/studies/S-EPMC6946545biostudies-literatureUnknown15(1)A poly (l-lactic) acid bioengineered anterior cruciate ligament (ACL) matrix has previously demonstrated the ability to support tissue regeneration in a rabbit ACL reconstruction model. The matrix was designed for optimal bone and ligament regeneration by developing a matrix with differential pore sizes in its bone and ligament compartments. Building upon past success, we designed a new bioengineered ACL matrix that is easier to install and can be used with endobutton fixation during ACL reconstruction. To achieve this, a new braiding procedure was developed to allow the matrix to be folded in half, making two-limbs, while maintaining its bone and ligament compartments. The osteointegration of the matrix with and without bone morphogenetic protein 2 (BMP-2) supplementation was evaluated inPloS oneEvaluation of a bioengineered ACL matrix's osteointegration with BMP-2 supplementation.PMC6946545DP1 AR068147R01AR063698R01 AR063698Mengsteab PYNair LSVella ATBadon MConroy PKan HMLaurencin CTOtsuka TfalseEvaluation of a bioengineered ACL matrix's osteointegration with BMP-2 supplementation.A poly (l-lactic) acid bioengineered anterior cruciate ligament (ACL) matrix has previously demonstrated the ability to support tissue regeneration in a rabbit ACL reconstruction model. The matrix was designed for optimal bone and ligament regeneration by developing a matrix with differential pore sizes in its bone and ligament compartments. Building upon past success, we designed a new bioengineered ACL matrix that is easier to install and can be used with endobutton fixation during ACL reconstruction. To achieve this, a new braiding procedure was developed to allow the matrix to be folded in half, making two-limbs, while maintaining its bone and ligament compartments. The osteointegration of the matrix with and without bone morphogenetic protein 2 (BMP-2) supplementation was evaluated in2020-01-01T00:00:00Z20202025-05-31T22:40:30.537Z2025-05-31T22:40:30.537ZS-EPMC69465453191023110.1371/journal.pone.0227181