{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lau MJ"],"funding":["Wellcome Trust"],"pagination":["223-231"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6947783"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["102(1)"],"pubmed_abstract":["Host seeking is an essential process in mosquito reproduction. Field releases of modified mosquitoes for population replacement rely on successful host seeking by female mosquitoes, but host-seeking ability is rarely tested in a realistic context. We tested the host-seeking ability of female <i>Aedes aegypti</i> mosquitoes using a semi-field system. Females with different <i>Wolbachia</i> infection types (<i>w</i>Mel-, <i>w</i>AlbB-infected, and uninfected) or from different origins (laboratory and field) were released at one end of a semi-field cage and recaptured as they landed on human experimenters 15 m away. Mosquitoes from each population were then identified with molecular tools or through minimal dusting with fluorescent powder. <i>Wolbachia</i>-infected and uninfected populations had similar average durations to landing and overall recapture proportions, as did laboratory and field-sourced <i>Ae. aegypti.</i> These results indicate that the host-seeking ability of mosquitoes is not negatively affected by <i>Wolbachia</i> infection or long-term laboratory maintenance. This method provides an approach to study the host-seeking ability of mosquitoes in a realistic setting, which will be useful when evaluating strains of mosquitoes that are planned for releases into the field to suppress arbovirus transmission."],"journal":["The American journal of tropical medicine and hygiene"],"pubmed_title":["Measuring the Host-Seeking Ability of <i>Aedes aegypti</i> Destined for Field Release."],"pmcid":["PMC6947783"],"funding_grant_id":["108508"],"pubmed_authors":["Lau MJ","Hoffmann AA","Ross PA","Ritchie SA","Axford JK","Endersby-Harshman NM"],"additional_accession":[]},"is_claimable":false,"name":"Measuring the Host-Seeking Ability of <i>Aedes aegypti</i> Destined for Field Release.","description":"Host seeking is an essential process in mosquito reproduction. Field releases of modified mosquitoes for population replacement rely on successful host seeking by female mosquitoes, but host-seeking ability is rarely tested in a realistic context. We tested the host-seeking ability of female <i>Aedes aegypti</i> mosquitoes using a semi-field system. Females with different <i>Wolbachia</i> infection types (<i>w</i>Mel-, <i>w</i>AlbB-infected, and uninfected) or from different origins (laboratory and field) were released at one end of a semi-field cage and recaptured as they landed on human experimenters 15 m away. Mosquitoes from each population were then identified with molecular tools or through minimal dusting with fluorescent powder. <i>Wolbachia</i>-infected and uninfected populations had similar average durations to landing and overall recapture proportions, as did laboratory and field-sourced <i>Ae. aegypti.</i> These results indicate that the host-seeking ability of mosquitoes is not negatively affected by <i>Wolbachia</i> infection or long-term laboratory maintenance. This method provides an approach to study the host-seeking ability of mosquitoes in a realistic setting, which will be useful when evaluating strains of mosquitoes that are planned for releases into the field to suppress arbovirus transmission.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jan","modification":"2021-02-20T17:17:54Z","creation":"2021-02-20T17:17:54Z"},"accession":"S-EPMC6947783","cross_references":{"pubmed":["31769394"],"doi":["10.4269/ajtmh.19-0510"]}}