biostudies-literatureBibbo' SAssociazione Italiana per la Ricerca sul CancroAssociazione Italiana per la Lotta al NeuroblastomaAssociazione Italiana per la Ricerca sul Cancro (Italian Association for Cancer Research)3https://www.ebi.ac.uk/biostudies/studies/S-EPMC6949307biostudies-literatureUnknown9(1)The MYC family of transcription factors is a major driver of human cancer and potential therapeutic target. However, no clinically viable drugs have been yet developed that are able to directly tackle MYC oncoproteins. In our laboratory, we are exploring alternative approaches aiming to disturb signalling downstream of MYC. MYCN is frequently activated in neuroblastoma, a paediatric solid malignancy that, in its metastatic form, has a very poor prognosis. An important pathway regulated by MYC is the CKS1/SKP2/p27^kip1 axis. In this study, we have repurposed the anti-psychotic drug Prozac to disrupt CKS1/SKP2/p27^Kip1 signalling and assess its potential as an anti-neuroblastoma agent in vitro and in vivo. Using DNA editing technology, we show that stabilisation of p27<suOncogenesisRepurposing a psychoactive drug for children with cancer: p27^Kip1-dependent inhibition of metastatic neuroblastomas by Prozac.PMC6949307IG:18467IG 15196Capone ESala GSallese MTsakaneli ACiufici PIezzi MPanella VSala ANieddu VLamolinara APurgato SDe Laurenzi VPerini GBibbo' SRossi CfalseRepurposing a psychoactive drug for children with cancer: p27^Kip1-dependent inhibition of metastatic neuroblastomas by Prozac.The MYC family of transcription factors is a major driver of human cancer and potential therapeutic target. However, no clinically viable drugs have been yet developed that are able to directly tackle MYC oncoproteins. In our laboratory, we are exploring alternative approaches aiming to disturb signalling downstream of MYC. MYCN is frequently activated in neuroblastoma, a paediatric solid malignancy that, in its metastatic form, has a very poor prognosis. An important pathway regulated by MYC is the CKS1/SKP2/p27^kip1 axis. In this study, we have repurposed the anti-psychotic drug Prozac to disrupt CKS1/SKP2/p27^Kip1 signalling and assess its potential as an anti-neuroblastoma agent in vitro and in vivo. Using DNA editing technology, we show that stabilisation of p27<su2020-01-01T00:00:00Z2020 Jan2025-04-25T18:47:05.559Z2020-05-22T07:23:49ZS-EPMC69493073190039910.1038/s41389-019-0186-3