biostudies-literatureUnknown118(1)Garcia XAgence Nationale de la RechercheAmong the many factors influencing fibrin formation and structure (concentration, temperature, composition, pH, etc.), it has been suggested that the polydispersity of fibrinogen may play an important role. We propose here a detailed investigation of the influence of this parameter on fibrin multiscale structure. Two commercial fibrinogen preparations were used, a monodisperse and a polydisperse one. First, the respective compositions of both fibrinogen preparations were thoroughly determined by measuring the fibrin-stabilizing factor; fibronectin; α, β, and γ intact chain contents; the γ/γ' chains ratio; the N-glycosylation; and the post-translational modifications. Slight variations between the composition of the two fibrinogen preparations were found that are much smaller than the compoBiophysical journal172-181https://www.ebi.ac.uk/biostudies/studies/S-EPMC6950636biostudies-literatureAggregates Dramatically Alter Fibrin Ultrastructure.PMC6950636Tellier ZCaton FGarcia XBihoreau NChevreux GSeyve LPolack BfalseAggregates Dramatically Alter Fibrin Ultrastructure.Among the many factors influencing fibrin formation and structure (concentration, temperature, composition, pH, etc.), it has been suggested that the polydispersity of fibrinogen may play an important role. We propose here a detailed investigation of the influence of this parameter on fibrin multiscale structure. Two commercial fibrinogen preparations were used, a monodisperse and a polydisperse one. First, the respective compositions of both fibrinogen preparations were thoroughly determined by measuring the fibrin-stabilizing factor; fibronectin; α, β, and γ intact chain contents; the γ/γ' chains ratio; the N-glycosylation; and the post-translational modifications. Slight variations between the composition of the two fibrinogen preparations were found that are much smaller than the compo2020-01-01T00:00:00Z2020 Jan2025-04-04T14:59:23.017Z2021-02-20T19:13:56ZS-EPMC69506363173532610.1016/j.bpj.2019.10.034