biostudies-literatureZhang YNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaE415https://www.ebi.ac.uk/biostudies/studies/S-EPMC6955681biostudies-literatureUnknown10(12)RNA interference (RNAi) has emerged as a novel and feasible strategy for pest management. Methods for cost-effective production and stable delivery of double-stranded RNA (dsRNA) to the target insects are crucial for the wide application of RNAi for pest control. In this study, we tested the expression of dsRNA in RNaseIII-deficient Escherichia coli HT115 which was then fed to Plagiodera versicolora larvae, an insect pest of Salicaceae plants worldwide. By targeting six potential genes, including actin (ACT), signal recognition particle protein 54k (SRP54), heat shock protein 70 (HSC70), shibire (SHI), cactus (CACT), and soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAP), we found that feeding bacteria-expressed dsRNA successfully triggered the silencing of the five targeInsectsBacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae).PMC6955681319716632017YFD060010131700227Xu LLi SZhang JZhang YfalseBacteria-Mediated RNA Interference for Management of Plagiodera versicolora (Coleoptera: Chrysomelidae).RNA interference (RNAi) has emerged as a novel and feasible strategy for pest management. Methods for cost-effective production and stable delivery of double-stranded RNA (dsRNA) to the target insects are crucial for the wide application of RNAi for pest control. In this study, we tested the expression of dsRNA in RNaseIII-deficient Escherichia coli HT115 which was then fed to Plagiodera versicolora larvae, an insect pest of Salicaceae plants worldwide. By targeting six potential genes, including actin (ACT), signal recognition particle protein 54k (SRP54), heat shock protein 70 (HSC70), shibire (SHI), cactus (CACT), and soluble N-ethylmaleimide-sensitive fusion attachment proteins (SNAP), we found that feeding bacteria-expressed dsRNA successfully triggered the silencing of the five targe2019-01-01T00:00:00Z2019 Nov2025-04-26T02:59:42.887Z2020-05-22T08:16:00ZS-EPMC69556813176638410.3390/insects10120415