{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(12)"],"submitter":["Li T"],"funding":["Hainan University world-class discipline construction funds","the Scientific Research Foundation of Hainan University","the National Natural Science Foundation of China"],"pubmed_abstract":["<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) is the causal agent of rice bacterial blight disease, which causes a large reduction in rice production. The successful interaction of pathogens and plants requires a particular nutrient environment that allows pathogen growth and the initiation of both pathogen and host responses. Amino acid synthesis is essential for bacterial growth when bacteria encounter amino acid-deficient environments, but the effects of amino acid synthesis on <i>Xoo</i> pathogenicity are unclear. Here, we systemically deleted the essential genes (<i>leuB</i>, <i>leuC</i>, <i>leuD</i>, <i>ilvC</i>, <i>thrC</i>, <i>hisD</i>, <i>trpC</i>, <i>argH</i>, <i>metB</i>, and <i>aspC</i>) involved in the synthesis of different amino acids and analyzed the effects of these mutations on <i>Xoo</i> virulence. Our results showed that leucine, isoleucine, valine, histidine, threonine, arginine, tryptophan, and cysteine syntheses are essential to <i>Xoo</i> infection. We further studied the role of leucine in the interaction between pathogens and hosts and found that leucine could stimulate some virulence-related responses and regulate <i>Xoo</i> pathogenicity. Our findings highlight that amino acids not only act as nutrients for bacterial growth but also play essential roles in the <i>Xoo</i> and rice interaction."],"journal":["Microorganisms"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6956189"],"repository":["biostudies-literature"],"pubmed_title":["Biosynthesis of Amino Acids in <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> Is Essential to Its Pathogenicity."],"pmcid":["PMC6956189"],"funding_grant_id":["31860485","kyqd1547","31660507","kyqd1546","RZZX201903"],"pubmed_authors":["Tao J","He C","Li C","Li T","Lin Y","Chen Y","Xie Q","Zhan Z","Lin M"],"additional_accession":[]},"is_claimable":false,"name":"Biosynthesis of Amino Acids in <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> Is Essential to Its Pathogenicity.","description":"<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) is the causal agent of rice bacterial blight disease, which causes a large reduction in rice production. The successful interaction of pathogens and plants requires a particular nutrient environment that allows pathogen growth and the initiation of both pathogen and host responses. Amino acid synthesis is essential for bacterial growth when bacteria encounter amino acid-deficient environments, but the effects of amino acid synthesis on <i>Xoo</i> pathogenicity are unclear. Here, we systemically deleted the essential genes (<i>leuB</i>, <i>leuC</i>, <i>leuD</i>, <i>ilvC</i>, <i>thrC</i>, <i>hisD</i>, <i>trpC</i>, <i>argH</i>, <i>metB</i>, and <i>aspC</i>) involved in the synthesis of different amino acids and analyzed the effects of these mutations on <i>Xoo</i> virulence. Our results showed that leucine, isoleucine, valine, histidine, threonine, arginine, tryptophan, and cysteine syntheses are essential to <i>Xoo</i> infection. We further studied the role of leucine in the interaction between pathogens and hosts and found that leucine could stimulate some virulence-related responses and regulate <i>Xoo</i> pathogenicity. Our findings highlight that amino acids not only act as nutrients for bacterial growth but also play essential roles in the <i>Xoo</i> and rice interaction.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Dec","modification":"2021-02-20T18:46:24Z","creation":"2020-05-22T08:16:39Z"},"accession":"S-EPMC6956189","cross_references":{"pubmed":["31847108"],"doi":["10.3390/microorganisms7120693"]}}