biostudies-literatureUnknown7(12)Li THainan University world-class discipline construction fundsthe Scientific Research Foundation of Hainan Universitythe National Natural Science Foundation of China<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) is the causal agent of rice bacterial blight disease, which causes a large reduction in rice production. The successful interaction of pathogens and plants requires a particular nutrient environment that allows pathogen growth and the initiation of both pathogen and host responses. Amino acid synthesis is essential for bacterial growth when bacteria encounter amino acid-deficient environments, but the effects of amino acid synthesis on <i>Xoo</i> pathogenicity are unclear. Here, we systemically deleted the essential genes (<i>leuB</i>, <i>leuC</i>, <i>leuD</i>, <i>ilvC</i>, <i>thrC</i>, <i>hisD</i>, <i>trpC</i>, <i>argH</i>, <i>metB</i>, and <i>aspC</i>) involved in the synthesis of different amino acids and analyzed the effects of these mutations on <i>Xoo</i> virulence. Our results showed that leucine, isoleucine, valine, histidine, threonine, arginine, tryptophan, and cysteine syntheses are essential to <i>Xoo</i> infection. We further studied the role of leucine in the interaction between pathogens and hosts and found that leucine could stimulate some virulence-related responses and regulate <i>Xoo</i> pathogenicity. Our findings highlight that amino acids not only act as nutrients for bacterial growth but also play essential roles in the <i>Xoo</i> and rice interaction.Microorganismshttps://www.ebi.ac.uk/biostudies/studies/S-EPMC6956189biostudies-literatureBiosynthesis of Amino Acids in <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> Is Essential to Its Pathogenicity.PMC695618931860485kyqd154731660507kyqd1546RZZX201903Tao JHe CLi CLi TLin YChen YXie QZhan ZLin MfalseBiosynthesis of Amino Acids in <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> Is Essential to Its Pathogenicity.<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> (<i>Xoo</i>) is the causal agent of rice bacterial blight disease, which causes a large reduction in rice production. The successful interaction of pathogens and plants requires a particular nutrient environment that allows pathogen growth and the initiation of both pathogen and host responses. Amino acid synthesis is essential for bacterial growth when bacteria encounter amino acid-deficient environments, but the effects of amino acid synthesis on <i>Xoo</i> pathogenicity are unclear. Here, we systemically deleted the essential genes (<i>leuB</i>, <i>leuC</i>, <i>leuD</i>, <i>ilvC</i>, <i>thrC</i>, <i>hisD</i>, <i>trpC</i>, <i>argH</i>, <i>metB</i>, and <i>aspC</i>) involved in the synthesis of different amino acids and analyzed the effects of these mutations on <i>Xoo</i> virulence. Our results showed that leucine, isoleucine, valine, histidine, threonine, arginine, tryptophan, and cysteine syntheses are essential to <i>Xoo</i> infection. We further studied the role of leucine in the interaction between pathogens and hosts and found that leucine could stimulate some virulence-related responses and regulate <i>Xoo</i> pathogenicity. Our findings highlight that amino acids not only act as nutrients for bacterial growth but also play essential roles in the <i>Xoo</i> and rice interaction.2019-01-01T00:00:00Z2019 Dec2021-02-20T18:46:24Z2020-05-22T08:16:39ZS-EPMC69561893184710810.3390/microorganisms7120693