{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(4)"],"submitter":["Crossley L"],"funding":["British Council Institutional Links grant","Engineering and Physical Sciences Research Council"],"pubmed_abstract":["We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open-circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and noncomplementary DNA target sequences."],"journal":["Biosensors"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6956290"],"repository":["biostudies-literature"],"pubmed_title":["Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)-An Electrochemical Optimisation Approach."],"pmcid":["PMC6956290"],"funding_grant_id":["EP/L015595/1","1813890","20180209"],"pubmed_authors":["Hannah S","Vezza V","Attoye B","Blair E","Crossley L","Corrigan DK"],"additional_accession":[]},"is_claimable":false,"name":"Establishing a Field-Effect Transistor Sensor for the Detection of Mutations in the Tumour Protein 53 Gene (TP53)-An Electrochemical Optimisation Approach.","description":"We present a low-cost, sensitive and specific DNA field-effect transistor sensor for the rapid detection of a common mutation to the tumour protein 53 gene (TP53). The sensor consists of a commercially available, low-cost, field-effect transistor attached in series to a gold electrode sensing pad for DNA hybridisation. The sensor has been predominantly optimised electrochemically, particularly with respect to open-circuit potentiometry as a route towards understanding potential (voltage) changes upon DNA hybridisation using a transistor. The developed sensor responds sensitively to TP53 mutant DNA as low as 100 nM concentration. The sensor responds linearly as a function of DNA target concentration and is able to differentiate between complementary and noncomplementary DNA target sequences.","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 Dec","modification":"2021-02-20T22:57:59Z","creation":"2020-05-22T08:16:47Z"},"accession":"S-EPMC6956290","cross_references":{"pubmed":["31817717"],"doi":["10.3390/bios9040141"]}}