biostudies-literatureSoncini SRNational Institute of Allergy and Infectious Diseases45https://www.ebi.ac.uk/biostudies/studies/S-EPMC6958937biostudies-literatureUnknown21(1)<h4>Background</h4>Clostridium perfringens is a Gram-positive anaerobic pathogen that causes multiple diseases in humans and animals. C. perfringens lack flagella but have type IV pili (TFP) and can glide on agar surfaces. When C. perfringens bacteria are placed on surfaces, they become elongated, flexible and have TFP on their surface, traits not seen in liquid-grown cells. In addition, the main pilin in C. perfringens TFP, PilA2, undergoes differential post-translational modification when grown in liquid or on plates. To understand the mechanisms underlying these phenotypes, bacteria were grown in three types of liquid media and on agar plates with the same medium to compare gene expression using RNA-Seq.<h4>Results</h4>Hundreds of genes were differentially expressed, including transcripBMC genomicsChanges in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces.PMC6958937R21AI1093Hartman AHJensen RVCamper GJGallagher TMMelville SBSoncini SRfalseChanges in the expression of genes encoding type IV pili-associated proteins are seen when Clostridium perfringens is grown in liquid or on surfaces.<h4>Background</h4>Clostridium perfringens is a Gram-positive anaerobic pathogen that causes multiple diseases in humans and animals. C. perfringens lack flagella but have type IV pili (TFP) and can glide on agar surfaces. When C. perfringens bacteria are placed on surfaces, they become elongated, flexible and have TFP on their surface, traits not seen in liquid-grown cells. In addition, the main pilin in C. perfringens TFP, PilA2, undergoes differential post-translational modification when grown in liquid or on plates. To understand the mechanisms underlying these phenotypes, bacteria were grown in three types of liquid media and on agar plates with the same medium to compare gene expression using RNA-Seq.<h4>Results</h4>Hundreds of genes were differentially expressed, including transcrip2020-01-01T00:00:00Z2020 Jan2026-04-29T16:01:21.777Z2020-05-22T08:31:09ZS-EPMC69589373193723710.1186/s12864-020-6453-z