{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Konopova B"],"funding":["Balfour-Browne Fund","Human Frontier Science Program","Isaac Newton Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6966819"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17"],"pubmed_abstract":["<h4>Background</h4>Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of \"orders\". They are missing in the genetic model <i>Drosophila</i> and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a \"hatching enzyme\" that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing.<h4>Results</h4>We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust <i>Schistocerca gregaria</i> (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis"],"journal":["Frontiers in zoology"],"pubmed_title":["Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch."],"pmcid":["PMC6966819"],"funding_grant_id":["not applicable","LT000733/2009-L","BB/ K009133/1","BB/K009133/1"],"pubmed_authors":["Buchberger E","Konopova B","Crisp A"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch.","description":"<h4>Background</h4>Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of \"orders\". They are missing in the genetic model <i>Drosophila</i> and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a \"hatching enzyme\" that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing.<h4>Results</h4>We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust <i>Schistocerca gregaria</i> (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-05-01T04:52:53.798Z","creation":"2020-05-22T08:29:59Z"},"accession":"S-EPMC6966819","cross_references":{"pubmed":["31969926"],"doi":["10.1186/s12983-019-0349-2"]}}