biostudies-literatureKonopova BBalfour-Browne FundHuman Frontier Science ProgramIsaac Newton TrustBiotechnology and Biological Sciences Research Council4https://www.ebi.ac.uk/biostudies/studies/S-EPMC6966819biostudies-literatureUnknown17<h4>Background</h4>Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of "orders". They are missing in the genetic model <i>Drosophila</i> and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a "hatching enzyme" that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing.<h4>Results</h4>We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust <i>Schistocerca gregaria</i> (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesisFrontiers in zoologyTranscriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch.PMC6966819not applicableLT000733/2009-LBB/ K009133/1BB/K009133/1Buchberger EKonopova BCrisp AfalseTranscriptome of pleuropodia from locust embryos supports that these organs produce enzymes enabling the larva to hatch.<h4>Background</h4>Pleuropodia are limb-derived glandular organs that transiently appear on the first abdominal segment in embryos of insects from majority of "orders". They are missing in the genetic model <i>Drosophila</i> and little is known about them. Experiments carried out on orthopteran insects 80 years ago indicated that the pleuropodia secrete a "hatching enzyme" that digests the serosal cuticle to enable the larva to hatch, but evidence by state-of-the-art molecular methods is missing.<h4>Results</h4>We used high-throughput RNA-sequencing to identify the genes expressed in the pleuropodia of the locust <i>Schistocerca gregaria</i> (Orthoptera). First, using transmission electron microscopy we studied the development of the pleuropodia during 11 stages of the locust embryogenesis2020-01-01T00:00:00Z20202026-05-01T04:52:53.798Z2020-05-22T08:29:59ZS-EPMC69668193196992610.1186/s12983-019-0349-2