biostudies-literatureCouttolenc-Brenis EConsejo Nacional de Ciencia y TecnologíaCONACYTInstitutional fundinge8345https://www.ebi.ac.uk/biostudies/studies/S-EPMC6982411biostudies-literatureUnknown8<h4>Background</h4>In Mexico, coffee leaf rust (CLR) is the main disease that affects the Arabica coffee crop. In this study, the local response of two Mexican cultivars of <i>Coffea arabica</i> (Oro Azteca and Garnica) in the early stages of <i>Hemileia vastatrix</i> infection was evaluated.<h4>Methods</h4>We quantified the development of fungal structures in locally-infected leaf disks from both cultivars, using qRT-PCR to measure the relative expression of two pathogenesis recognition genes (<i>CaNDR1</i> and <i>CaNBS-LRR</i>) and three genes associated with the salicylic acid (SA)-related pathway (<i>CaNPR1</i>, <i>CaPR1,</i> and <i>CaPR5</i>).<h4>Results</h4>Resistance of the cv. Oro Azteca was significantly higher than that of the cv. Garnica, with 8.2% and 53.3% haustorial detectionPeerJPrehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes.PMC6982411CB-2014, 242999LN-2017, 280505Villain LMata-Rosas MCarrion GLCouttolenc-Brenis EOrtega-Escalona FRamirez-Martinez DMendez-Bravo AfalsePrehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes.<h4>Background</h4>In Mexico, coffee leaf rust (CLR) is the main disease that affects the Arabica coffee crop. In this study, the local response of two Mexican cultivars of <i>Coffea arabica</i> (Oro Azteca and Garnica) in the early stages of <i>Hemileia vastatrix</i> infection was evaluated.<h4>Methods</h4>We quantified the development of fungal structures in locally-infected leaf disks from both cultivars, using qRT-PCR to measure the relative expression of two pathogenesis recognition genes (<i>CaNDR1</i> and <i>CaNBS-LRR</i>) and three genes associated with the salicylic acid (SA)-related pathway (<i>CaNPR1</i>, <i>CaPR1,</i> and <i>CaPR5</i>).<h4>Results</h4>Resistance of the cv. Oro Azteca was significantly higher than that of the cv. Garnica, with 8.2% and 53.3% haustorial detection2020-01-01T00:00:00Z20202025-05-29T22:01:48.536Z2025-05-29T22:01:48.536ZS-EPMC69824113200232710.7717/peerj.8345