biostudies-literatureSong JAgricultural Science and Technology Innovation ProgramNational Key Technology R&D ProgramChina Agriculture Research Systemskz396https://www.ebi.ac.uk/biostudies/studies/S-EPMC6986778biostudies-literatureUnknown98(1)We investigated the effects of inulin on intestinal barrier function and mucosal immunity in Salmonella enterica serovar Enteritidis (SE)-infected specific pathogen-free (SPF) chickens. SPF chickens (n = 240, 1-d-old) were divided into 4 groups (6 replicates per group, 10 chickens per replicate): a control group (CON) fed a basal diet without inulin supplementation and 3 SE-infected groups fed a basal diet supplemented with inulin 0% (SE group), 0.5% (0.5% InSE group), and 1% (1% InSE group), respectively. At 28 d of age, the chickens in SE-infected groups were orally infected with SE and in CON group were administrated with phosphated-buffered saline (PBS). Intestinal morphology, mucosal immunity, and intestinal barrier function-related gene expression were analyzed at 1- and 3-d post-infJournal of animal scienceEffects of inulin supplementation on intestinal barrier function and immunity in specific pathogen-free chickens with Salmonella infection.PMC6986778ASTIPIAS042015BAD03B03CARS-41CAAS-XTCX2016010-03Wen JZheng MZhao GLi QLiu REveraert NSong JfalseEffects of inulin supplementation on intestinal barrier function and immunity in specific pathogen-free chickens with Salmonella infection.We investigated the effects of inulin on intestinal barrier function and mucosal immunity in Salmonella enterica serovar Enteritidis (SE)-infected specific pathogen-free (SPF) chickens. SPF chickens (n = 240, 1-d-old) were divided into 4 groups (6 replicates per group, 10 chickens per replicate): a control group (CON) fed a basal diet without inulin supplementation and 3 SE-infected groups fed a basal diet supplemented with inulin 0% (SE group), 0.5% (0.5% InSE group), and 1% (1% InSE group), respectively. At 28 d of age, the chickens in SE-infected groups were orally infected with SE and in CON group were administrated with phosphated-buffered saline (PBS). Intestinal morphology, mucosal immunity, and intestinal barrier function-related gene expression were analyzed at 1- and 3-d post-inf2020-01-01T00:00:00Z2020 Jan2025-04-04T22:27:09.243Z2021-02-20T18:01:08ZS-EPMC69867783189424110.1093/jas/skz396