{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Aztekin C"],"funding":["Cancer Research UK","Medical Research Council","Wellcome Trust"],"pagination":["653-658"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6986927"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["364(6441)"],"pubmed_abstract":["Unlike mammals, <i>Xenopus laevis</i> tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key r"],"journal":["Science (New York, N.Y.)"],"pubmed_title":["Identification of a regeneration-organizing cell in the <i>Xenopus</i> tail."],"pmcid":["PMC6986927"],"funding_grant_id":["101050/Z/13/Z","098357","101050","105031","098357/Z/12/Z","092096","MC_PC_12009","22231","105031/Z/14/Z","092096/Z/10/Z","A14492"],"pubmed_authors":["Gurdon JB","Jullien J","Aztekin C","Hiscock TW","Simons BD","Marioni JC"],"additional_accession":[]},"is_claimable":false,"name":"Identification of a regeneration-organizing cell in the <i>Xenopus</i> tail.","description":"Unlike mammals, <i>Xenopus laevis</i> tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key r","dates":{"release":"2019-01-01T00:00:00Z","publication":"2019 May","modification":"2025-04-18T15:51:46.484Z","creation":"2020-10-29T16:05:55Z"},"accession":"S-EPMC6986927","cross_references":{"pubmed":["31097661"],"doi":["10.1126/science.aav9996"]}}