biostudies-literatureRowe MNICHD NIH HHSSyracuse UniversityResearch Council of NorwayMarilyn Kerr Fellowship488-506https://www.ebi.ac.uk/biostudies/studies/S-EPMC6993853biostudies-literatureUnknown37(2)Seminal fluid proteins (SFPs) mediate an array of postmating reproductive processes that influence fertilization and fertility. As such, it is widely held that SFPs may contribute to postmating, prezygotic reproductive barriers between closely related taxa. We investigated seminal fluid (SF) diversification in a recently diverged passerine species pair (Passer domesticus and Passer hispaniolensis) using a combination of proteomic and comparative evolutionary genomic approaches. First, we characterized and compared the SF proteome of the two species, revealing consistencies with known aspects of SFP biology and function in other taxa, including the presence and diversification of proteins involved in immunity and sperm maturation. Second, using whole-genome resequencing data, we assessed paMolecular biology and evolutionMolecular Diversification of the Seminal Fluid Proteome in a Recently Diverged Passerine Species Pair.PMC6993853R21 HD088910230434Whittington EEroukhmanoff FSætre GPDorus SRowe MBorziak KRavinet MfalseMolecular Diversification of the Seminal Fluid Proteome in a Recently Diverged Passerine Species Pair.Seminal fluid proteins (SFPs) mediate an array of postmating reproductive processes that influence fertilization and fertility. As such, it is widely held that SFPs may contribute to postmating, prezygotic reproductive barriers between closely related taxa. We investigated seminal fluid (SF) diversification in a recently diverged passerine species pair (Passer domesticus and Passer hispaniolensis) using a combination of proteomic and comparative evolutionary genomic approaches. First, we characterized and compared the SF proteome of the two species, revealing consistencies with known aspects of SFP biology and function in other taxa, including the presence and diversification of proteins involved in immunity and sperm maturation. Second, using whole-genome resequencing data, we assessed pa2020-01-01T00:00:00Z2020 Feb2026-04-08T06:08:10.085Z2020-05-22T09:31:18ZS-EPMC69938533166551010.1093/molbev/msz235