{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dong S"],"funding":["NIA NIH HHS","NIDDK NIH HHS","NCI NIH HHS","NINDS NIH HHS"],"pagination":["645"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6994528"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["Autophagy malfunctioning occurs in multiple human disorders, making attractive the idea of chemically modulating it with therapeutic purposes. However, for many types of autophagy, a clear understanding of tissue-specific differences in their activity and regulation is missing because of lack of methods to monitor these processes in vivo. Chaperone-mediated autophagy (CMA) is a selective type of autophagy that until now has only been studied in vitro and not in the tissue context at single cell resolution. Here, we develop a transgenic reporter mouse that allows dynamic measurement of CMA activity in vivo using image-based procedures. We identify previously unknown spatial and temporal differences in CMA activity in multiple organs and in response to stress. We illustrate the versatility o"],"journal":["Nature communications"],"pubmed_title":["Monitoring spatiotemporal changes in chaperone-mediated autophagy in vivo."],"pmcid":["PMC6994528"],"funding_grant_id":["P30 AG038072","R01 DK124308","U54 NS100717","RF1 AG054108","R37 AG021904","K22 CA196750","P01 AG031782","R01 DK098408"],"pubmed_authors":["Scrivo A","Cuervo AM","Bravo-Cordero JJ","Eliscovich C","Aguirre-Hernandez C","Arias E","Dong S"],"additional_accession":[]},"is_claimable":false,"name":"Monitoring spatiotemporal changes in chaperone-mediated autophagy in vivo.","description":"Autophagy malfunctioning occurs in multiple human disorders, making attractive the idea of chemically modulating it with therapeutic purposes. However, for many types of autophagy, a clear understanding of tissue-specific differences in their activity and regulation is missing because of lack of methods to monitor these processes in vivo. Chaperone-mediated autophagy (CMA) is a selective type of autophagy that until now has only been studied in vitro and not in the tissue context at single cell resolution. Here, we develop a transgenic reporter mouse that allows dynamic measurement of CMA activity in vivo using image-based procedures. We identify previously unknown spatial and temporal differences in CMA activity in multiple organs and in response to stress. We illustrate the versatility o","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jan","modification":"2025-04-27T03:58:42.845Z","creation":"2020-05-22T09:31:54Z"},"accession":"S-EPMC6994528","cross_references":{"pubmed":["32005807"],"doi":["10.1038/s41467-019-14164-4"]}}