biostudies-literatureDong SNIA NIH HHSNIDDK NIH HHSNCI NIH HHSNINDS NIH HHS645https://www.ebi.ac.uk/biostudies/studies/S-EPMC6994528biostudies-literatureUnknown11(1)Autophagy malfunctioning occurs in multiple human disorders, making attractive the idea of chemically modulating it with therapeutic purposes. However, for many types of autophagy, a clear understanding of tissue-specific differences in their activity and regulation is missing because of lack of methods to monitor these processes in vivo. Chaperone-mediated autophagy (CMA) is a selective type of autophagy that until now has only been studied in vitro and not in the tissue context at single cell resolution. Here, we develop a transgenic reporter mouse that allows dynamic measurement of CMA activity in vivo using image-based procedures. We identify previously unknown spatial and temporal differences in CMA activity in multiple organs and in response to stress. We illustrate the versatility oNature communicationsMonitoring spatiotemporal changes in chaperone-mediated autophagy in vivo.PMC6994528P30 AG038072R01 DK124308U54 NS100717RF1 AG054108R37 AG021904K22 CA196750P01 AG031782R01 DK098408Scrivo ACuervo AMBravo-Cordero JJEliscovich CAguirre-Hernandez CArias EDong SfalseMonitoring spatiotemporal changes in chaperone-mediated autophagy in vivo.Autophagy malfunctioning occurs in multiple human disorders, making attractive the idea of chemically modulating it with therapeutic purposes. However, for many types of autophagy, a clear understanding of tissue-specific differences in their activity and regulation is missing because of lack of methods to monitor these processes in vivo. Chaperone-mediated autophagy (CMA) is a selective type of autophagy that until now has only been studied in vitro and not in the tissue context at single cell resolution. Here, we develop a transgenic reporter mouse that allows dynamic measurement of CMA activity in vivo using image-based procedures. We identify previously unknown spatial and temporal differences in CMA activity in multiple organs and in response to stress. We illustrate the versatility o2020-01-01T00:00:00Z2020 Jan2025-04-27T03:58:42.845Z2020-05-22T09:31:54ZS-EPMC69945283200580710.1038/s41467-019-14164-4