biostudies-literatureLee QNIAID NIH HHSNCI NIH HHSe1008262https://www.ebi.ac.uk/biostudies/studies/S-EPMC6999912biostudies-literatureUnknown16(1)Mouse kidney parvovirus (MKPV) is a member of the provisional genus Chapparvovirus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four major MKPV transcripts, created by alternative splicing, to a common initiator region, and use mass spectrometry to identify "p10" and "p15" as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and the splicing-dependent putative accessory protein NS2 are conserved in all near-complete amniote chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropiPLoS pathogensMurine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys.PMC6999912P30 CA008748U19 AI109761Lee QBrenner OPinello NPadula MPShaban BMonette SCrim MJRoediger BWong JJO'Rourke MBJolly CJWeninger WSong RSouza WMPimanda JEWilliams SHFumagalli MJOrkin JDMelin ADfalseMurine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys.Mouse kidney parvovirus (MKPV) is a member of the provisional genus Chapparvovirus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four major MKPV transcripts, created by alternative splicing, to a common initiator region, and use mass spectrometry to identify "p10" and "p15" as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and the splicing-dependent putative accessory protein NS2 are conserved in all near-complete amniote chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropi2020-01-01T00:00:00Z2020 Jan2026-05-07T00:38:07.089Z2020-05-22T10:32:32ZS-EPMC69999123197197910.1371/journal.ppat.1008262