biostudies-literatureEar JNCINIAID NIH HHSNCI NIH HHSNIHNIGMS NIH HHS100859https://www.ebi.ac.uk/biostudies/studies/S-EPMC7005484biostudies-literatureUnknown23(2)Polarized distribution of organelles and molecules inside a cell is vital for a range of cellular processes and its loss is frequently encountered in disease. Polarization during planar cell migration is a special condition in which cellular orientation is triggered by cell-cell contact. We demonstrate that the protein Daple (CCDC88C) is a component of cell junctions in epithelial cells which serves like a cellular "compass" for establishing and maintaining contact-triggered planar polarity. Furthermore, these processes may be mediated through interaction with the polarity regulator PARD3. This interaction, mediated by Daple's PDZ-binding motif (PBM) and the third PDZ domain of PARD3, is fine-tuned by tyrosine phosphorylation on Daple's PBM by receptor and non-receptor tyrosine kinases, suiScienceTyrosine-Based Signals Regulate the Assembly of Daple⋅PARD3 Complex at Cell-Cell Junctions.PMC7005484R01 CA238042R01 GM071872CA238042R01 CA160911T32 CA067754R01 GM117424R01 AI118985R01 CA100768GM071872GM117424CA100768AI118985CA160911Rajapakse NKufareva IGhosh PEar JGhassemian MChoi JSaklecha AfalseTyrosine-Based Signals Regulate the Assembly of Daple⋅PARD3 Complex at Cell-Cell Junctions.Polarized distribution of organelles and molecules inside a cell is vital for a range of cellular processes and its loss is frequently encountered in disease. Polarization during planar cell migration is a special condition in which cellular orientation is triggered by cell-cell contact. We demonstrate that the protein Daple (CCDC88C) is a component of cell junctions in epithelial cells which serves like a cellular "compass" for establishing and maintaining contact-triggered planar polarity. Furthermore, these processes may be mediated through interaction with the polarity regulator PARD3. This interaction, mediated by Daple's PDZ-binding motif (PBM) and the third PDZ domain of PARD3, is fine-tuned by tyrosine phosphorylation on Daple's PBM by receptor and non-receptor tyrosine kinases, su2020-01-01T00:00:00Z2020 Feb2026-05-07T06:46:53.807Z2020-05-22T10:07:44ZS-EPMC70054843205897010.1016/j.isci.2020.100859