biostudies-literatureUnknown8(1)Hettiarachchige IKMethods for the identification and localisation of endophytic fungi are required to study the establishment, development, and progression of host-symbiont interactions, as visible reactions or disease symptoms are generally absent from host plants. Fluorescent proteins have proved valuable as reporter gene products, allowing non-invasive detection in living cells. This study reports the introduction of genes for two fluorescent proteins, green fluorescent protein (GFP) and red fluorescent protein, DsRed, into the genomes of two distinct perennial ryegrass (<i>Lolium perenne</i> L.)-associated <i>Epichloë</i> endophyte strains using <i>A. tumefaciens</i>-mediated transformation. Comprehensive characterisation of reporter gene-containing endophyte strains was performed using molecular genetiMicroorganismsE54https://www.ebi.ac.uk/biostudies/studies/S-EPMC7023320biostudies-literatureGeneration of <i>Epichloe</i> Strains Expressing Fluorescent Proteins Suitable for Studying Host-Endophyte Interactions and Characterisation of a T-DNA Integration Event.PMC7023320Hettiarachchige IKSpangenberg GCGuthridge KMBrohier NDSawbridge TILudlow EJSahab SEkanayake PNfalseGeneration of <i>Epichloe</i> Strains Expressing Fluorescent Proteins Suitable for Studying Host-Endophyte Interactions and Characterisation of a T-DNA Integration Event.Methods for the identification and localisation of endophytic fungi are required to study the establishment, development, and progression of host-symbiont interactions, as visible reactions or disease symptoms are generally absent from host plants. Fluorescent proteins have proved valuable as reporter gene products, allowing non-invasive detection in living cells. This study reports the introduction of genes for two fluorescent proteins, green fluorescent protein (GFP) and red fluorescent protein, DsRed, into the genomes of two distinct perennial ryegrass (<i>Lolium perenne</i> L.)-associated <i>Epichloë</i> endophyte strains using <i>A. tumefaciens</i>-mediated transformation. Comprehensive characterisation of reporter gene-containing endophyte strains was performed using molecular geneti2019-01-01T00:00:00Z2019 Dec2026-04-08T03:27:03.414Z2020-05-22T12:28:53ZS-EPMC70233203189217310.3390/microorganisms8010054