biostudies-literatureMir MAKing Saud University975-984https://www.ebi.ac.uk/biostudies/studies/S-EPMC7042633biostudies-literatureUnknown27(3)Saffron plant (<i>Crocus sativus</i> L.) is being used as a source of saffron spice and medicine to cure or prevent different types of diseases including cancers. We report the isolation, characterization of bioactive small molecule ([crocetin (β-d-glucosyl) ester] from the leaf biowastes of saffron plant of Kashmir, India. MTTC assay and Bio-autography aided approach were used to assess anti-oxidant activity and anti-cancer properties of crocin (s) against DPPH free radical and breast cancer cell line respectively. Crocetin beta-d-glucosyl ester restrained proliferation of human breast adeno-carcinoma cell model (MCF-7) without significantly affecting normal cell line (L-6). Further studies involving molecular mechanics generalized born surface area and molecular docking showed that croceSaudi journal of biological sciencesIsolation, purification and characterization of naturally derived Crocetin beta-d-glucosyl ester from <i>Crocus sativus</i> L. against breast cancer and its binding chemistry with ER-alpha/HDAC2.PMC7042633RSP-2019/116Mani PMasoodi KZRehman MUAhmad PAmin HJan SGanai SAMansoor SMir MAfalseIsolation, purification and characterization of naturally derived Crocetin beta-d-glucosyl ester from <i>Crocus sativus</i> L. against breast cancer and its binding chemistry with ER-alpha/HDAC2.Saffron plant (<i>Crocus sativus</i> L.) is being used as a source of saffron spice and medicine to cure or prevent different types of diseases including cancers. We report the isolation, characterization of bioactive small molecule ([crocetin (β-d-glucosyl) ester] from the leaf biowastes of saffron plant of Kashmir, India. MTTC assay and Bio-autography aided approach were used to assess anti-oxidant activity and anti-cancer properties of crocin (s) against DPPH free radical and breast cancer cell line respectively. Crocetin beta-d-glucosyl ester restrained proliferation of human breast adeno-carcinoma cell model (MCF-7) without significantly affecting normal cell line (L-6). Further studies involving molecular mechanics generalized born surface area and molecular docking showed that croce2020-01-01T00:00:00Z2020 Mar2025-04-20T02:04:02.546Z2020-05-22T12:03:08ZS-EPMC70426333212777710.1016/j.sjbs.2020.01.018