{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ma W"],"funding":["National Natural Science Foundation of China"],"pagination":["42"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7045492"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(1)"],"pubmed_abstract":["<h4>Background</h4>The CDKN2A/B locus contains crucial tumor suppressors and a lncRNA gene ANRIL. However, the mechanisms that coordinately regulate their expression levels are not clear.<h4>Methods</h4>Novel RNAs transcribed from the CDKN2A gene were screened by CDKN2A-specific RNA capture deep-sequencing and confirmed by Northern blotting and clone-sequencing. Long non-coding RNA (lncRNA) binding proteins were characterized by RNA pull-down combined with mass spectrometry and RNA immunoprecipitation. LncRNA functions in human cells were studied using a set of biological assays in vitro and in vivo.<h4>Results</h4>We characterized a novel lncRNA, P14AS with its promoter in the antisense strand of the fragment near CDKN2A exon 1b in human cells. The mature P14AS is a three-exon linear cyto"],"journal":["Molecular cancer"],"pubmed_title":["Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells."],"pmcid":["PMC7045492"],"funding_grant_id":["91640108"],"pubmed_authors":["Deng D","Gu L","Qiao J","Ma W","Zhou J"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells.","description":"<h4>Background</h4>The CDKN2A/B locus contains crucial tumor suppressors and a lncRNA gene ANRIL. However, the mechanisms that coordinately regulate their expression levels are not clear.<h4>Methods</h4>Novel RNAs transcribed from the CDKN2A gene were screened by CDKN2A-specific RNA capture deep-sequencing and confirmed by Northern blotting and clone-sequencing. Long non-coding RNA (lncRNA) binding proteins were characterized by RNA pull-down combined with mass spectrometry and RNA immunoprecipitation. LncRNA functions in human cells were studied using a set of biological assays in vitro and in vivo.<h4>Results</h4>We characterized a novel lncRNA, P14AS with its promoter in the antisense strand of the fragment near CDKN2A exon 1b in human cells. The mature P14AS is a three-exon linear cyto","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Feb","modification":"2026-04-16T19:07:09.98Z","creation":"2020-05-22T11:44:46Z"},"accession":"S-EPMC7045492","cross_references":{"pubmed":["32106863"],"doi":["10.1186/s12943-020-01150-4"]}}