biostudies-literatureMa WNational Natural Science Foundation of China42https://www.ebi.ac.uk/biostudies/studies/S-EPMC7045492biostudies-literatureUnknown19(1)<h4>Background</h4>The CDKN2A/B locus contains crucial tumor suppressors and a lncRNA gene ANRIL. However, the mechanisms that coordinately regulate their expression levels are not clear.<h4>Methods</h4>Novel RNAs transcribed from the CDKN2A gene were screened by CDKN2A-specific RNA capture deep-sequencing and confirmed by Northern blotting and clone-sequencing. Long non-coding RNA (lncRNA) binding proteins were characterized by RNA pull-down combined with mass spectrometry and RNA immunoprecipitation. LncRNA functions in human cells were studied using a set of biological assays in vitro and in vivo.<h4>Results</h4>We characterized a novel lncRNA, P14AS with its promoter in the antisense strand of the fragment near CDKN2A exon 1b in human cells. The mature P14AS is a three-exon linear cytoMolecular cancerCharacterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells.PMC704549291640108Deng DGu LQiao JMa WZhou JfalseCharacterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells.<h4>Background</h4>The CDKN2A/B locus contains crucial tumor suppressors and a lncRNA gene ANRIL. However, the mechanisms that coordinately regulate their expression levels are not clear.<h4>Methods</h4>Novel RNAs transcribed from the CDKN2A gene were screened by CDKN2A-specific RNA capture deep-sequencing and confirmed by Northern blotting and clone-sequencing. Long non-coding RNA (lncRNA) binding proteins were characterized by RNA pull-down combined with mass spectrometry and RNA immunoprecipitation. LncRNA functions in human cells were studied using a set of biological assays in vitro and in vivo.<h4>Results</h4>We characterized a novel lncRNA, P14AS with its promoter in the antisense strand of the fragment near CDKN2A exon 1b in human cells. The mature P14AS is a three-exon linear cyto2020-01-01T00:00:00Z2020 Feb2026-04-16T19:07:09.98Z2020-05-22T11:44:46ZS-EPMC70454923210686310.1186/s12943-020-01150-4