{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hiebert CW"],"funding":["Gouvernement du Canada | Agriculture and Agri-Food Canada","National Science Foundation (NSF)","Commonwealth Scientific and Industrial Research Organisation","Biotechnology and Biological Sciences Research Council","Western Grains Research Foundation","National Science Foundation"],"pagination":["1123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7048732"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming"],"journal":["Nature communications"],"pubmed_title":["Stem rust resistance in wheat is suppressed by a subunit of the mediator complex."],"pmcid":["PMC7048732"],"funding_grant_id":["BBS/E/J/000PR9795","IOS-0965429","BB/P012574/1","BB/J003166/1","BB/P016855/1","BB/L011794/1","BBS/E/J/000CA562","BBS/E/J/000PR9780","BBS/E/J/000C0673"],"pubmed_authors":["Moscou MJ","Zhang J","Steuernagel B","Vrana J","Rouse MN","Huang L","Hiebert CW","Jin Y","Spielmeyer W","Upadhyaya N","Zhang P","Fetch T","Wulff BBH","Green P","McIntosh RA","Karafiatova M","Hernandez-Pinzon I","Bhavani S","Dolezel J","Lagudah E","Hewitt T","Pujol V"],"additional_accession":[]},"is_claimable":false,"name":"Stem rust resistance in wheat is suppressed by a subunit of the mediator complex.","description":"Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Feb","modification":"2026-05-02T22:08:25.648Z","creation":"2020-05-22T11:38:48Z"},"accession":"S-EPMC7048732","cross_references":{"pubmed":["32111840"],"doi":["10.1038/s41467-020-14937-2"]}}