biostudies-literatureHiebert CWGouvernement du Canada | Agriculture and Agri-Food CanadaNational Science Foundation (NSF)Commonwealth Scientific and Industrial Research OrganisationBiotechnology and Biological Sciences Research CouncilWestern Grains Research FoundationNational Science Foundation1123https://www.ebi.ac.uk/biostudies/studies/S-EPMC7048732biostudies-literatureUnknown11(1)Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogrammingNature communicationsStem rust resistance in wheat is suppressed by a subunit of the mediator complex.PMC7048732BBS/E/J/000PR9795IOS-0965429BB/P012574/1BB/J003166/1BB/P016855/1BB/L011794/1BBS/E/J/000CA562BBS/E/J/000PR9780BBS/E/J/000C0673Moscou MJZhang JSteuernagel BVrana JRouse MNHuang LHiebert CWJin YSpielmeyer WUpadhyaya NZhang PFetch TWulff BBHGreen PMcIntosh RAKarafiatova MHernandez-Pinzon IBhavani SDolezel JLagudah EHewitt TPujol VfalseStem rust resistance in wheat is suppressed by a subunit of the mediator complex.Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming2020-01-01T00:00:00Z2020 Feb2026-05-02T22:08:25.648Z2020-05-22T11:38:48ZS-EPMC70487323211184010.1038/s41467-020-14937-2