biostudies-literatureVijaykumar ANIDCR NIH HHSNational Institute of Health104647https://www.ebi.ac.uk/biostudies/studies/S-EPMC7050286biostudies-literatureUnknown111<h4>Objective</h4>In the present study, we compared the in vitro osteogenic and dentinogenic potential of pulp cells from incisors and molars.<h4>Design</h4>Primary pulp cultures were established from DSPP-Cerulean/DMP1-Cherry and BSP-GFPtpz reporter mouse lines and processed for various assays.<h4>Results</h4>Our results showed marked differences in dentinogenic and osteogenic potentials of primary cultures from unerupted molars and incisors isolated from 5 to 7 days old pups. While primary cultures from both incisors and molars differentiated into odontoblasts and osteoblasts, cultures from molars differentiated into more DSPP-Cerulean + cells (∼5.5 %) compared to incisor cultures (∼0.7 %) at Day 14 and appear to be more committed to the odontogenic lineage. On the other hand, cultures fArchives of oral biologyComparison of osteogenic and dentinogenic potentials of mice incisor and molar pulps in vitro.PMC7050286R90 DE022526T90 DE021989R01 DE016689R01-DE016689T90-DE022526Vijaykumar AMina MfalseComparison of osteogenic and dentinogenic potentials of mice incisor and molar pulps in vitro.<h4>Objective</h4>In the present study, we compared the in vitro osteogenic and dentinogenic potential of pulp cells from incisors and molars.<h4>Design</h4>Primary pulp cultures were established from DSPP-Cerulean/DMP1-Cherry and BSP-GFPtpz reporter mouse lines and processed for various assays.<h4>Results</h4>Our results showed marked differences in dentinogenic and osteogenic potentials of primary cultures from unerupted molars and incisors isolated from 5 to 7 days old pups. While primary cultures from both incisors and molars differentiated into odontoblasts and osteoblasts, cultures from molars differentiated into more DSPP-Cerulean + cells (∼5.5 %) compared to incisor cultures (∼0.7 %) at Day 14 and appear to be more committed to the odontogenic lineage. On the other hand, cultures f2020-01-01T00:00:00Z2020 Mar2025-04-04T19:15:02.333Z2021-03-03T08:08:51ZS-EPMC70502863195865810.1016/j.archoralbio.2019.104647