{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stack TMM"],"funding":["NCRR NIH HHS","National Institute of General Medical Sciences","NIGMS NIH HHS","Salisbury University"],"pagination":["1657-1661"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7059403"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["142(4)"],"pubmed_abstract":["l-Ascorbate (vitamin C) is ubiquitous in both our diet and the environment. Here we report that <i>Ralstonia eutropha</i> H16 (<i>Cupriavidus necator</i> ATCC 17699) uses l-ascorbate as sole carbon source via a novel catabolic pathway. RNaseq identified eight candidate catabolic genes, sequence similarity networks, and genome neighborhood networks guided predictions for function of the encoded proteins, and the predictions were confirmed by <i>in vitro</i> assays and <i>in vivo</i> growth phenotypes of gene deletion mutants. l-Ascorbate, a lactone, is oxidized and ring-opened by enzymes in the cytochrome <i>b</i><sub>561</sub> and gluconolactonase families, respectively, to form 2,3-diketo-l-gulonate. A protein predicted to have a WD40-like fold catalyzes an unprecedented benzilic acid rea"],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Characterization of an l-Ascorbate Catabolic Pathway with Unprecedented Enzymatic Transformations."],"pmcid":["PMC7059403"],"funding_grant_id":["S10 RR028833","P01GM118303","P01 GM118303"],"pubmed_authors":["Dettmer TM","Kim C","Jermain M","Morrison KN","Gerlt JA","Stack TMM","Joyce R","Wille B","Bhatti K","Francisco BS","Naing YT","Carter MS"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of an l-Ascorbate Catabolic Pathway with Unprecedented Enzymatic Transformations.","description":"l-Ascorbate (vitamin C) is ubiquitous in both our diet and the environment. Here we report that <i>Ralstonia eutropha</i> H16 (<i>Cupriavidus necator</i> ATCC 17699) uses l-ascorbate as sole carbon source via a novel catabolic pathway. RNaseq identified eight candidate catabolic genes, sequence similarity networks, and genome neighborhood networks guided predictions for function of the encoded proteins, and the predictions were confirmed by <i>in vitro</i> assays and <i>in vivo</i> growth phenotypes of gene deletion mutants. l-Ascorbate, a lactone, is oxidized and ring-opened by enzymes in the cytochrome <i>b</i><sub>561</sub> and gluconolactonase families, respectively, to form 2,3-diketo-l-gulonate. A protein predicted to have a WD40-like fold catalyzes an unprecedented benzilic acid rea","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jan","modification":"2026-04-18T19:13:26.52Z","creation":"2021-02-21T04:12:03Z"},"accession":"S-EPMC7059403","cross_references":{"pubmed":["31917558"],"doi":["10.1021/jacs.9b09863"]}}