{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang J"],"funding":["the Joint-innovation Program in Healthcare for Special Scientific Research Projects of Guangzhou","the International Collaboration Program of Natural Science Foundation of China and US NIH","Natural Science Foundation of Jilin Province","the National Special Research Program of China for Important Infectious Diseases","the National Natural Science Foundation of China"],"pagination":["e1008334"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7062283"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(2)"],"pubmed_abstract":["Influenza A virus (IAV) infection is a complicated process. After IAVs spread to the lung, extensive pro-inflammatory cytokines and chemokines are released, which largely determine the outcome of infection. Using a single-cell RNA sequencing (scRNA-seq) assay, we systematically and sequentially analyzed the transcriptome of more than 16,000 immune cells in the pulmonary tissue of infected mice, and demonstrated that two waves of pro-inflammatory factors were released. A group of IAV-infected PD-L1+ neutrophils were the major contributor to the first wave at an earlier stage (day 1-3 post infection). Notably, at a later stage (day 7 post infection) when IAV was hardly detected in the immune cells, a group of platelet factor 4-positive (Pf4+)-macrophages generated another wave of pro-inflammatory factors, which were probably the precursors of alveolar macrophages (AMs). Furthermore, single-cell signaling map identified inter-lineage crosstalk between different clusters and helped better understand the signature of PD-L1+ neutrophils and Pf4+-macrophages. Our data characteristically clarified the infiltrated immune cells and their production of pro-inflammatory factors during the immunopathogenesis development, and deciphered the important mechanisms underlying IAV-driven inflammatory reactions in the lung."],"journal":["PLoS pathogens"],"pubmed_title":["Two waves of pro-inflammatory factors are released during the influenza A virus (IAV)-driven pulmonary immunopathogenesis."],"pmcid":["PMC7062283"],"funding_grant_id":["2018ZX10101004003001","201803040002","81701990","2018ZX10302103, 2017ZX10202102-003","81601759","81730060","81561128007"],"pubmed_authors":["Huang F","Liu J","Ma R","Xi Z","Pan T","Zhang J","Zhang H","Deng K","Luo B","Wang J","Zhao M","Lu G","Zhang Y","Zhang X","Liu B","Luo Y","Yuan Y"],"additional_accession":[]},"is_claimable":false,"name":"Two waves of pro-inflammatory factors are released during the influenza A virus (IAV)-driven pulmonary immunopathogenesis.","description":"Influenza A virus (IAV) infection is a complicated process. After IAVs spread to the lung, extensive pro-inflammatory cytokines and chemokines are released, which largely determine the outcome of infection. Using a single-cell RNA sequencing (scRNA-seq) assay, we systematically and sequentially analyzed the transcriptome of more than 16,000 immune cells in the pulmonary tissue of infected mice, and demonstrated that two waves of pro-inflammatory factors were released. A group of IAV-infected PD-L1+ neutrophils were the major contributor to the first wave at an earlier stage (day 1-3 post infection). Notably, at a later stage (day 7 post infection) when IAV was hardly detected in the immune cells, a group of platelet factor 4-positive (Pf4+)-macrophages generated another wave of pro-inflammatory factors, which were probably the precursors of alveolar macrophages (AMs). Furthermore, single-cell signaling map identified inter-lineage crosstalk between different clusters and helped better understand the signature of PD-L1+ neutrophils and Pf4+-macrophages. Our data characteristically clarified the infiltrated immune cells and their production of pro-inflammatory factors during the immunopathogenesis development, and deciphered the important mechanisms underlying IAV-driven inflammatory reactions in the lung.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Feb","modification":"2026-04-15T23:24:18.688Z","creation":"2020-05-22T13:51:03Z"},"accession":"S-EPMC7062283","cross_references":{"pubmed":["32101596"],"doi":["10.1371/journal.ppat.1008334"]}}