biostudies-literatureScornec HEuropean Research CouncilEuropean Regional Development Fund156https://www.ebi.ac.uk/biostudies/studies/S-EPMC7062640biostudies-literatureUnknown11The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (<i>cwaR</i>), LSEI_0220 (<i>cwaS</i>), and LSEI_0221 (<i>ldcA</i>), has been previously identified as required for the establishment of <i>Lactobacillus paracasei</i> in the gut. The genes <i>cwaR</i> and <i>cwaS</i> encode a predicted two-component system (TCS) and <i>ldcA</i> a predicted D-alanyl-D-alanine carboxypeptidase which is a peptidoglycan (PG) biosynthesis enzyme. We explored the functionality and the physiological role of these three genes, particularly their impact on the bacterial cell wall architecture and on the bacterial adaptation to environmental perturbations in the gut. The functionality of CwaS/R proteins as a TCS has been demonstrated by biochemicFrontiers in microbiologyStudy of the <i>cwaRS-ldcA</i> Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in <i>Lactobacillus paracasei</i>.PMC7062640202283232798Boneca IGSansonetti PJPedron TWheeler RPalud AScornec HLicandro HPetitgonnet CCavin JFfalseStudy of the <i>cwaRS-ldcA</i> Operon Coding a Two-Component System and a Putative L,D-Carboxypeptidase in <i>Lactobacillus paracasei</i>.The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (<i>cwaR</i>), LSEI_0220 (<i>cwaS</i>), and LSEI_0221 (<i>ldcA</i>), has been previously identified as required for the establishment of <i>Lactobacillus paracasei</i> in the gut. The genes <i>cwaR</i> and <i>cwaS</i> encode a predicted two-component system (TCS) and <i>ldcA</i> a predicted D-alanyl-D-alanine carboxypeptidase which is a peptidoglycan (PG) biosynthesis enzyme. We explored the functionality and the physiological role of these three genes, particularly their impact on the bacterial cell wall architecture and on the bacterial adaptation to environmental perturbations in the gut. The functionality of CwaS/R proteins as a TCS has been demonstrated by biochemic2020-01-01T00:00:00Z20202026-05-05T04:47:43.181Z2020-05-22T13:43:23ZS-EPMC70626403219451010.3389/fmicb.2020.00156