biostudies-literatureSogawa CSuzuken Memorial FoundationRyobi Teien Memory FoundationJapan Society for the Promotion of ScienceE523https://www.ebi.ac.uk/biostudies/studies/S-EPMC7072357biostudies-literatureUnknown12(2)Tumor growth, progression, and therapy resistance are crucial factors in the prognosis of cancer. The properties of three-dimensional (3D) tumor-like organoids (tumoroids) more closely resemble in vivo tumors compared to two-dimensionally cultured cells and are therefore effectively used for assays and drug screening. We here established a repurposed drug for novel anticancer research and therapeutics using a 3D tumoroid-based screening system. We screened six pharmacologically active compounds by using an original tumoroid-based multiplex phenotypic screening system with a matrix metalloproteinase 9 (MMP9) promoter-driven fluorescence reporter for the evaluation of both tumoroid formation and progression. The antiparkinson drug benztropine was the most effective compound uncovered by the CancersAntiparkinson Drug Benztropine Suppresses Tumor Growth, Circulating Tumor Cells, and Metastasis by Acting on SLC6A3/DAT and Reducing STAT3.PMC7072357CSTEKO17K11643JP17K11642JP17K11669JP17K17895JP17K1168919H0381719H04051JP18K09789Trin KTran MTTaha EAIshige MSogawa CKawai HLu YOkamoto KSogawa NCalderwood SKOkusha YKozaki KIEguchi TTakigawa MfalseAntiparkinson Drug Benztropine Suppresses Tumor Growth, Circulating Tumor Cells, and Metastasis by Acting on SLC6A3/DAT and Reducing STAT3.Tumor growth, progression, and therapy resistance are crucial factors in the prognosis of cancer. The properties of three-dimensional (3D) tumor-like organoids (tumoroids) more closely resemble in vivo tumors compared to two-dimensionally cultured cells and are therefore effectively used for assays and drug screening. We here established a repurposed drug for novel anticancer research and therapeutics using a 3D tumoroid-based screening system. We screened six pharmacologically active compounds by using an original tumoroid-based multiplex phenotypic screening system with a matrix metalloproteinase 9 (MMP9) promoter-driven fluorescence reporter for the evaluation of both tumoroid formation and progression. The antiparkinson drug benztropine was the most effective compound uncovered by the 2020-01-01T00:00:00Z2020 Feb2025-04-26T01:06:20.327Z2020-05-22T13:28:38ZS-EPMC70723573210244010.3390/cancers12020523