biostudies-literatureYu T‘‘Reintroduction Technologies and Demonstration of Extremely Rare Wild Plant Population’’ of National Key Research and Development ProgramMinistry of Science and Technology of Taiwan409-418https://www.ebi.ac.uk/biostudies/studies/S-EPMC7078402biostudies-literatureUnknown26(3)<i>Thuja sutchuenensis</i> is a critically endangered tertiary relict species of Cupressaceae from southwestern China. We sequenced the complete chloroplast (cp) genome of <i>T</i>. <i>sutchuenensis</i>, showing the genome content of 129,776 bp, 118 unique genes including 82 unique protein-coding genes, 32 tRNA genes, and 4 rRNA genes. The genome structures, gene order, and GC content are similar to other typical gymnosperm cp genomes. Thirty-eight simple sequence repeats were identified in the <i>T</i>. <i>sutchuenensis</i> cp genome. We also found an apparent inversion between <i>trnT</i> and <i>psbK</i> between genera <i>Thuja</i> and <i>Thujopsis</i>. In addition, positive selection signals were detected in seven genes with high Ka/Ks ratios. The reconstructed phylogeny based on locallPhysiology and molecular biology of plants : an international journal of functional plant biologyChloroplast genome of an extremely endangered conifer <i>Thuja sutchuenensis</i> Franch.: gene organization, comparative and phylogenetic analysis.PMC7078402MOST 105–2628-B-003–002-MY32016YFC0503106MOST 105–2628-B-003–001-MY3Li JQZhang YHuang BHLiao PCYu TfalseChloroplast genome of an extremely endangered conifer <i>Thuja sutchuenensis</i> Franch.: gene organization, comparative and phylogenetic analysis.<i>Thuja sutchuenensis</i> is a critically endangered tertiary relict species of Cupressaceae from southwestern China. We sequenced the complete chloroplast (cp) genome of <i>T</i>. <i>sutchuenensis</i>, showing the genome content of 129,776 bp, 118 unique genes including 82 unique protein-coding genes, 32 tRNA genes, and 4 rRNA genes. The genome structures, gene order, and GC content are similar to other typical gymnosperm cp genomes. Thirty-eight simple sequence repeats were identified in the <i>T</i>. <i>sutchuenensis</i> cp genome. We also found an apparent inversion between <i>trnT</i> and <i>psbK</i> between genera <i>Thuja</i> and <i>Thujopsis</i>. In addition, positive selection signals were detected in seven genes with high Ka/Ks ratios. The reconstructed phylogeny based on locall2020-01-01T00:00:00Z2020 Mar2026-04-30T17:10:24.905Z2021-03-03T08:08:47ZS-EPMC70784023220591910.1007/s12298-019-00736-7