{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Krichel B"],"funding":["European Research Council"],"pagination":["1009-1019"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7078746"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["477(5)"],"pubmed_abstract":["Severe acute respiratory syndrome coronavirus is the causative agent of a respiratory disease with a high case fatality rate. During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Here, we analyzed processing of the coronavirus nsp7-10 region using native mass spectrometry showing consumption of substrate, rise and fall of intermediate products and complexation. Importantly, there is a clear order of cleavage efficiencies, which is influenced by the polyprotein tertiary structure. Furthermore, the predominant product is an nsp7+8(2 : 2) hetero-tetramer with nsp8 scaffold. In conclusion, native MS, opposed to ot"],"journal":["The Biochemical journal"],"pubmed_title":["Processing of the SARS-CoV pp1a/ab nsp7-10 region."],"pmcid":["PMC7078746"],"funding_grant_id":["759661"],"pubmed_authors":["Falke S","Hilgenfeld R","Redecke L","Uetrecht C","Krichel B"],"additional_accession":[]},"is_claimable":false,"name":"Processing of the SARS-CoV pp1a/ab nsp7-10 region.","description":"Severe acute respiratory syndrome coronavirus is the causative agent of a respiratory disease with a high case fatality rate. During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Here, we analyzed processing of the coronavirus nsp7-10 region using native mass spectrometry showing consumption of substrate, rise and fall of intermediate products and complexation. Importantly, there is a clear order of cleavage efficiencies, which is influenced by the polyprotein tertiary structure. Furthermore, the predominant product is an nsp7+8(2 : 2) hetero-tetramer with nsp8 scaffold. In conclusion, native MS, opposed to ot","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Mar","modification":"2026-05-01T16:18:51.953Z","creation":"2026-04-07T17:03:58.77Z"},"accession":"S-EPMC7078746","cross_references":{"pubmed":["32083638"],"doi":["10.1042/BCJ20200029"]}}