{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["21(5)"],"submitter":["Mano SS"],"funding":["Japan Society for the Promotion of Science"],"pubmed_abstract":["BACKGROUND:We propose the potential studies on material fluidity to induce epithelial to mesenchymal transition (EMT) in MCF-7 cells. In this study, we examined for the first time the effect of material fluidity on EMT using poly(?-caprolactone-co-D,L-lactide) (P(CL-co-DLLA)) with tunable elasticity and fluidity. METHODS:The fluidity was altered by chemically crosslinking the polymer networks. The crosslinked P(CL-co-DLLA) substrate showed a solid-like property with a stiffness of 261 kPa, while the non-crosslinked P(CL-co-DLLA) substrate of 100 units (high fluidity) and 500 units (low fluidity) existed in a quasi-liquid state with loss modulus of 33 kPa and 30.8 kPa, respectively, and storage modulus of 10.8 kPa and 20.1 kPa, respectively. RESULTS:We observed that MCF-7 cells on low fluidic substrates decreased the expression of E-cadherin, an epithelial marker, and increased expression of vimentin, a mesenchymal marker. This showed that the cells lose their epithelial phenotype and gain a mesenchymal property. On the other hand, MCF-7 cells on high fluidic substrates maintained their epithelial phenotype, suggesting that the cells did not undergo EMT. CONCLUSION:Considering these results as the fundamental information for material fluidity induced EMT, our system could be used to regulate the degree of EMT by turning the fluidity of the material."],"journal":["International journal of molecular sciences"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7084864"],"repository":["biostudies-literature"],"pubmed_title":["Fluidity of Poly (?-Caprolactone)-Based Material Induces Epithelial-to-Mesenchymal Transition."],"pmcid":["PMC7084864"],"funding_grant_id":["19H04476"],"pubmed_authors":["Ebara M","Mano SS","Uto K"],"additional_accession":[]},"is_claimable":false,"name":"Fluidity of Poly (?-Caprolactone)-Based Material Induces Epithelial-to-Mesenchymal Transition.","description":"BACKGROUND:We propose the potential studies on material fluidity to induce epithelial to mesenchymal transition (EMT) in MCF-7 cells. In this study, we examined for the first time the effect of material fluidity on EMT using poly(?-caprolactone-co-D,L-lactide) (P(CL-co-DLLA)) with tunable elasticity and fluidity. METHODS:The fluidity was altered by chemically crosslinking the polymer networks. The crosslinked P(CL-co-DLLA) substrate showed a solid-like property with a stiffness of 261 kPa, while the non-crosslinked P(CL-co-DLLA) substrate of 100 units (high fluidity) and 500 units (low fluidity) existed in a quasi-liquid state with loss modulus of 33 kPa and 30.8 kPa, respectively, and storage modulus of 10.8 kPa and 20.1 kPa, respectively. RESULTS:We observed that MCF-7 cells on low fluidic substrates decreased the expression of E-cadherin, an epithelial marker, and increased expression of vimentin, a mesenchymal marker. This showed that the cells lose their epithelial phenotype and gain a mesenchymal property. On the other hand, MCF-7 cells on high fluidic substrates maintained their epithelial phenotype, suggesting that the cells did not undergo EMT. CONCLUSION:Considering these results as the fundamental information for material fluidity induced EMT, our system could be used to regulate the degree of EMT by turning the fluidity of the material.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Mar","modification":"2020-11-19T11:09:48Z","creation":"2020-05-22T14:08:12Z"},"accession":"S-EPMC7084864","cross_references":{"pubmed":["32143443"],"doi":["10.3390/ijms21051757"]}}