biostudies-literatureIwanaka TKAKENHIKAKENI134https://www.ebi.ac.uk/biostudies/studies/S-EPMC7093986biostudies-literatureUnknown11(1)<h4>Background</h4>Human deciduous pulp stem cells (hDPSCs) have remarkable stem cell potency associated with cell proliferation, mesenchymal multipotency, and immunosuppressive function and have shown beneficial effects in a variety of animal disease models. Recent studies demonstrated that hDPSCs exhibited in vivo anti-fibrotic and anti-inflammatory action and in vivo hepatogenic-associated liver regeneration, suggesting that hDPSCs may offer a promising source with great clinical demand for treating liver diseases. However, how to manufacture ex vivo large-scale clinical-grade hDPSCs with the appropriate quality, safety, and preclinical efficacy assurances remains unclear.<h4>Methods</h4>We isolated hDPSCs from human deciduous dental pulp tissues formed by the colony-forming unit-fibrobStem cell research & therapyA model study for the manufacture and validation of clinical-grade deciduous dental pulp stem cells for chronic liver fibrosis treatment.PMC70939862529340517K1151318K0859816H02682Matsuura TYamaza TSonoda SIwanaka TYoshimaru KOda YYamaza HOhga STaguchi TfalseA model study for the manufacture and validation of clinical-grade deciduous dental pulp stem cells for chronic liver fibrosis treatment.<h4>Background</h4>Human deciduous pulp stem cells (hDPSCs) have remarkable stem cell potency associated with cell proliferation, mesenchymal multipotency, and immunosuppressive function and have shown beneficial effects in a variety of animal disease models. Recent studies demonstrated that hDPSCs exhibited in vivo anti-fibrotic and anti-inflammatory action and in vivo hepatogenic-associated liver regeneration, suggesting that hDPSCs may offer a promising source with great clinical demand for treating liver diseases. However, how to manufacture ex vivo large-scale clinical-grade hDPSCs with the appropriate quality, safety, and preclinical efficacy assurances remains unclear.<h4>Methods</h4>We isolated hDPSCs from human deciduous dental pulp tissues formed by the colony-forming unit-fibrob2020-01-01T00:00:00Z2020 Mar2025-04-18T11:51:18.572Z2020-05-22T14:37:04ZS-EPMC70939863221319810.1186/s13287-020-01630-w