biostudies-literatureOliai Araghi SMinisterie van Economische ZakenNederlandse Zuivel OrganisatieZonMw1253-1262https://www.ebi.ac.uk/biostudies/studies/S-EPMC7098930biostudies-literatureUnknown59(3)<h4>Purpose</h4>Higher folate and vitamin-B12 have been linked to lower risk of overweight. However, whether this is a causal effect of these B-vitamins on obesity risk remains unclear and evidence in older individuals is scarce. This study aimed to assess the role of B-vitamin supplementation and levels on body composition in older individuals.<h4>Methods</h4>A double-blind, randomized controlled trial in 2919 participants aged ≥ 65 years with elevated homocysteine levels. The intervention comprised a 2-year supplementation with a combination of folic acid (400 µg) and vitamin B12 (500 µg), or with placebo. Serum folate, vitamin-B12, active vitamin-B12 (HoloTC), methylmalonic acid (MMA), and anthropometrics were measured at baseline and after 2 years of follow-up. Dietary intake of folateEuropean journal of nutritionB-vitamins and body composition: integrating observational and experimental evidence from the B-PROOF study.PMC70989306130.0031project KB-15-004-003Kiefte-de Jong JCZillikens MCUitterlinden AGvan Schoor NMVoortman TOliai Araghi Svan Dijk SCStricker BHvan der Velde NBraun KVEde Groot LCPGMfalseB-vitamins and body composition: integrating observational and experimental evidence from the B-PROOF study.<h4>Purpose</h4>Higher folate and vitamin-B12 have been linked to lower risk of overweight. However, whether this is a causal effect of these B-vitamins on obesity risk remains unclear and evidence in older individuals is scarce. This study aimed to assess the role of B-vitamin supplementation and levels on body composition in older individuals.<h4>Methods</h4>A double-blind, randomized controlled trial in 2919 participants aged ≥ 65 years with elevated homocysteine levels. The intervention comprised a 2-year supplementation with a combination of folic acid (400 µg) and vitamin B12 (500 µg), or with placebo. Serum folate, vitamin-B12, active vitamin-B12 (HoloTC), methylmalonic acid (MMA), and anthropometrics were measured at baseline and after 2 years of follow-up. Dietary intake of folate2020-01-01T00:00:00Z2020 Apr2026-04-28T23:05:17.961Z2020-05-22T14:43:14ZS-EPMC70989303107685610.1007/s00394-019-01985-8