{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hatakeyama S"],"funding":["Ministry of Education, Culture, Sports, Science and Technology","National Institute of Biomedical Innovation","Japan Science and Technology Agency"],"pagination":["99-108"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7103410"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["380(1)"],"pubmed_abstract":["When expressed in mammalian cells, the nucleocapsid (N) and membrane (M) proteins of the severe acute respiratory syndrome coronavirus (SARS-CoV) are sufficient to form pseudoparticles. To identify region(s) of the N molecule required for pseudoparticle formation, we performed biochemical analysis of the interaction of N mutants and M in HEK293 cells. Using a peptide library derived from N, we found that amino acids 101-115 constituted a novel binding site for M. We examined the ability of N mutants to interact with M and form pseudoparticles, and our observations indicated that M bound to NDelta(101-115), N1-150, N151-300, and N301-422, but not to N1-150Delta(101-115). However, pseudoparticles were formed when NDelta(101-115) or N301-422, but not N1-150 or N151-300, were expressed with M "],"journal":["Virology"],"pubmed_title":["Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus."],"pmcid":["PMC7103410"],"funding_grant_id":["04-02"],"pubmed_authors":["Kanai T","Ishida I","Ueshiba H","Kirikae T","Matsuoka Y","Hatakeyama S","Itoh K","Komatsu N","Shichijo S","Sasazuki T","Miyoshi-Akiyama T","Fukushi M"],"additional_accession":[]},"is_claimable":false,"name":"Dissection and identification of regions required to form pseudoparticles by the interaction between the nucleocapsid (N) and membrane (M) proteins of SARS coronavirus.","description":"When expressed in mammalian cells, the nucleocapsid (N) and membrane (M) proteins of the severe acute respiratory syndrome coronavirus (SARS-CoV) are sufficient to form pseudoparticles. To identify region(s) of the N molecule required for pseudoparticle formation, we performed biochemical analysis of the interaction of N mutants and M in HEK293 cells. Using a peptide library derived from N, we found that amino acids 101-115 constituted a novel binding site for M. We examined the ability of N mutants to interact with M and form pseudoparticles, and our observations indicated that M bound to NDelta(101-115), N1-150, N151-300, and N301-422, but not to N1-150Delta(101-115). However, pseudoparticles were formed when NDelta(101-115) or N301-422, but not N1-150 or N151-300, were expressed with M ","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Oct","modification":"2026-05-07T07:28:22.5Z","creation":"2025-05-31T22:42:17.417Z"},"accession":"S-EPMC7103410","cross_references":{"pubmed":["18703211"],"doi":["10.1016/j.virol.2008.07.012"]}}