{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Billon P"],"funding":["Rally Foundation","NCI NIH HHS","NIH","Chemotherapy Foundation","NIGMS NIH HHS","Leukemia and Lymphoma Society"],"pagination":["3280-3295.e6"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7108696"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30(10)"],"pubmed_abstract":["Genome editing technologies have transformed our ability to engineer desired genomic changes within living systems. However, detecting precise genomic modifications often requires sophisticated, expensive, and time-consuming experimental approaches. Here, we describe DTECT (Dinucleotide signaTurE CapTure), a rapid and versatile detection method that relies on the capture of targeted dinucleotide signatures resulting from the digestion of genomic DNA amplicons by the type IIS restriction enzyme AcuI. DTECT enables the accurate quantification of marker-free precision genome editing events introduced by CRISPR-dependent homology-directed repair, base editing, or prime editing in various biological systems, such as mammalian cell lines, organoids, and tissues. Furthermore, DTECT allows the ide"],"journal":["Cell reports"],"pubmed_title":["Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures."],"pmcid":["PMC7108696"],"funding_grant_id":["F31 CA224800","R35 CA210065","P01 CA174653","R01 CA216981","120749-14","R01 CA197774","P30 CA008748","LLS 6531-18","R01 CA172272","R01 GM117064","P30 CA013696"],"pubmed_authors":["Nambiar TS","Dunbar A","Levine RL","Breinig M","Ciccia A","Schatoff EM","Park YC","Ryu HS","Dow LE","Zafra MP","Hayward SB","Oshima K","Baer R","Billon P","Tschaharganeh DF","Ferrando A"],"additional_accession":[]},"is_claimable":false,"name":"Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures.","description":"Genome editing technologies have transformed our ability to engineer desired genomic changes within living systems. However, detecting precise genomic modifications often requires sophisticated, expensive, and time-consuming experimental approaches. Here, we describe DTECT (Dinucleotide signaTurE CapTure), a rapid and versatile detection method that relies on the capture of targeted dinucleotide signatures resulting from the digestion of genomic DNA amplicons by the type IIS restriction enzyme AcuI. DTECT enables the accurate quantification of marker-free precision genome editing events introduced by CRISPR-dependent homology-directed repair, base editing, or prime editing in various biological systems, such as mammalian cell lines, organoids, and tissues. Furthermore, DTECT allows the ide","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Mar","modification":"2026-04-29T22:47:04.545Z","creation":"2020-05-22T14:54:29Z"},"accession":"S-EPMC7108696","cross_references":{"pubmed":["32160537"],"doi":["10.1016/j.celrep.2020.02.068"]}}