biostudies-literatureUnknown7(1)Li SFthe Natinal Natural Science Foundation of Chinathe National Natural Science Foundation of Chinathe Key Scientific Research Projects of Colleges and Universities in Henan Province<i>Asparagus setaceus</i> is a popular ornamental plant cultivated in tropical and subtropical regions globally. Here, we constructed a chromosome-scale reference genome of <i>A. setaceus</i> to facilitate the investigation of its genome characteristics and evolution. Using a combination of Nanopore long reads, Illumina short reads, 10× Genomics linked reads, and Hi-C data, we generated a high-quality genome assembly of <i>A. setaceus</i> covering 710.15 Mb, accounting for 98.63% of the estimated genome size. A total of 96.85% of the sequences were anchored to ten superscaffolds corresponding to the ten chromosomes. The genome of <i>A. setaceus</i> was predicted to contain 28,410 genes, 25,649 (90.28%) of which were functionally annotated. A total of 65.59% of the genome was occupied by reHorticulture research48https://www.ebi.ac.uk/biostudies/studies/S-EPMC7109074biostudies-literatureChromosome-level genome assembly, annotation and evolutionary analysis of the ornamental plant <i>Asparagus setaceus</i>.PMC7109074Lan LNDeng CLZhang YLLi SFZhu HWWang JDong RLi NGao WJfalseChromosome-level genome assembly, annotation and evolutionary analysis of the ornamental plant <i>Asparagus setaceus</i>.<i>Asparagus setaceus</i> is a popular ornamental plant cultivated in tropical and subtropical regions globally. Here, we constructed a chromosome-scale reference genome of <i>A. setaceus</i> to facilitate the investigation of its genome characteristics and evolution. Using a combination of Nanopore long reads, Illumina short reads, 10× Genomics linked reads, and Hi-C data, we generated a high-quality genome assembly of <i>A. setaceus</i> covering 710.15 Mb, accounting for 98.63% of the estimated genome size. A total of 96.85% of the sequences were anchored to ten superscaffolds corresponding to the ten chromosomes. The genome of <i>A. setaceus</i> was predicted to contain 28,410 genes, 25,649 (90.28%) of which were functionally annotated. A total of 65.59% of the genome was occupied by re2020-01-01T00:00:00Z20202026-05-04T17:23:48.921Z2025-05-29T19:04:09.445ZS-EPMC71090743225723410.1038/s41438-020-0271-y